{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["026:00"], "contactPoint": {"@type": "vcard:Contact", "fn": "GeneLab Outreach", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"}, "description": "The purpose of this study was to evaluate transcriptional changes in mouse spleens using a ground-based model for spaceflight. This model includes prolonged unloading and low-dose irradiation. Low-dose-rate gamma-radiation was delivered to 6-month old female C57BL/6J mice using 57Co plates (0.04 Gy) to simulate the radiation environment of spaceflight. Anti-orthostatic tail suspension was used to model the unloading fluid shift and physiological stress aspects of the microgravity component of spaceflight. Mice were hindlimb suspended and/or irradiated for 21 days. Mice were euthanized and spleens collected 7 days following treatment. RNA sequencing data was generated to assess transcriptional changes in these spleens.", "distribution": [{"@type": "dcat:Distribution", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-211", "format": "HTML", "mediaType": "text/html", "title": "Transcriptomic analysis of spleens from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both"}], "identifier": "nasa_genelab_GLDS-211_ye6w-xar9", "issued": "2021-05-21", "keyword": ["data-transformation", "genelab-rnaseq-data-processing-protocol", "hindlimb-unloading", "ionizing-radiation", "library-construction", "nucleic-acid-extraction", "nucleic-acid-sequencing", "sample-collection", "spike-in-protocol", "treatment-protocol"], "landingPage": "https://data.nasa.gov/dataset/transcriptomic-analysis-of-spleens-from-mice-subjected-to-chronic-low-dose-radiation-hindl", "license": "http://www.usa.gov/publicdomain/label/1.0/", "modified": "2025-04-23", "programCode": ["026:005"], "publisher": {"@type": "org:Organization", "name": "National Aeronautics and Space Administration"}, "theme": ["Earth Science"], "title": "Transcriptomic analysis of spleens from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both"}