{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Bearson, Bradley, L.", "hasEmail": "mailto:brad.bearson@usda.gov"}, "description": "<p>Conjugation efficiency of <em>Salmonella</em> Genomic Island 4 (SGI-4) from donor <em>Salmonella enterica</em> serovar I 4,[5],12:i:- strain BBS 1356 or a plasmid containing<em> oriT</em><sub>SGI-4_966</sub> from donor <em>Salmonella enterica</em> serovar I 4,[5],12:i:- strain BBS 1636 to recipient strains of <em>Salmonella enterica</em> serovars (Choleraesuis, Infantis, Enteritidis, and Senftenberg) or other Enterobacteriaceae (<em>Escherichia coli</em> and <em>Citrobacter youngae</em>)<em>.</em> The <em>oriT</em><sub>SGI-4_966</sub> nucleotide sequence spans nucleotides <a href=\"https://agdatacommons.nal.usda.gov/institution/review\" target=\"_blank\">4704810</a>-<a href=\"https://agdatacommons.nal.usda.gov/institution/review\" target=\"_blank\">4705775</a> of the <em>Salmonella enterica</em> serovar I 4,[5],12:i:- strain USDA15WA-1 genome (<a href=\"https://www.ncbi.nlm.nih.gov/nuccore/CP040686\" target=\"_blank\">CP040686</a>). Conjugation efficiency was calculated by dividing the colony forming units (CFU)/mL of transconjugants divided by the CFU/mL of donors. A transconjugant is a bacterial recipient that received DNA from the donor strain due to horizontal gene transfer.</p>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/49431946", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "HGT SGI-4 or oriTSGI-4_966 to Salmonella serovars and Enterobacteriaceae.xlsx"}], "identifier": "10.15482/USDA.ADC/27111475.v1", "keyword": ["Bacterial conjugation", "Horizontal gene transfer", "Salmonella", "Salmonella 4,[5],12:i:-", "Salmonella Genomic Island 4", "Salmonella enterica serovar I 4,[5],12:i:-", "integrative conjugative element", "origin of transfer (oriT)"], "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2026-05-27", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "temporal": "2023-10-01/2023-10-01", "title": "Horizontal Gene Transfer of SGI-4 (from BBS 1356) or a plasmid containing<i> oriT</i><sub>SGI-4_966</sub> (from BBS 1636) to <i>Salmonella</i> serovars or other Enterobacteriaceae"}