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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                        <gco:CharacterString>Institution web page</gco:CharacterString>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                <gmd:CI_Contact>
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                    <gmd:CI_OnlineResource>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                        <gco:CharacterString>Consolidated Safety Services-Dynamac (CSS) website</gco:CharacterString>
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                        <gco:CharacterString>Institution web page</gco:CharacterString>
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                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                        <gco:CharacterString>Institution web page</gco:CharacterString>
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                        <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
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              <gmd:organisationName>
                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                <gmd:CI_Contact>
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                    <gmd:CI_OnlineResource>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                        <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
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                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                      <gmd:name>
                        <gco:CharacterString>Consolidated Safety Services-Dynamac (CSS) website</gco:CharacterString>
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                        <gco:CharacterString>Institution web page</gco:CharacterString>
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                        <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
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                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                <gmd:CI_Contact>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                      <gmd:protocol>
                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                      <gmd:name>
                        <gco:CharacterString>Consolidated Safety Services-Dynamac (CSS) website</gco:CharacterString>
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                        <gco:CharacterString>Institution web page</gco:CharacterString>
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                        <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
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                <gmx:Anchor xlink:href="https://ror.org/03mz0by90" xlink:actuate="onRequest">Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                        <gmd:URL>https://www.css-inc.com/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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                        <gco:CharacterString>Consolidated Safety Services-Dynamac (CSS) website</gco:CharacterString>
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            <gmd:CI_ResponsibleParty>
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                <gmx:Anchor xlink:href="https://ror.org/05ba43f71" xlink:actuate="onRequest">NOAA National Centers for Coastal Ocean Science (NCCOS)</gmx:Anchor>
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                        <gmd:URL>https://coastalscience.noaa.gov/</gmd:URL>
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                <gmx:Anchor xlink:href="https://ror.org/033mqx355" xlink:actuate="onRequest">NOAA Fisheries (NMFS)</gmx:Anchor>
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                        <gmd:URL>https://www.fisheries.noaa.gov/</gmd:URL>
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                        <gco:CharacterString>HTTPS</gco:CharacterString>
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        <gco:CharacterString>The purpose of this work is to fill data gaps related to the effects of chronic turbidity exposure on Florida’s shallow-water stony corals. These new data are intended to be used in Florida’s efforts to improve water quality to ensure protection of Florida’s coral reefs. The data from the turbidity-elevated temperature study is intended to provide resource managers with information regarding possible additive or synergistic effects that can together lower safety thresholds for coral health. This provides actionable information for managing nearshore activities (dredging, construction, etc.) that create elevated turbid conditions for coral, particularly during periods of elevated temperatures.</gco:CharacterString>
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        <gco:CharacterString>Funding Agency: NOAA National Centers for Coastal Ocean Science (NCCOS)</gco:CharacterString>
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        <gco:CharacterString>Funding Agency: Coral Reef Conservation Program (CRCP)</gco:CharacterString>
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            <gmx:Anchor xlink:href="https://ror.org/04r0wrp59" xlink:actuate="onRequest">NOAA National Centers for Environmental Information</gmx:Anchor>
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                    <gmd:URL>https://www.ncei.noaa.gov/contact</gmd:URL>
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                    <gco:CharacterString>NCEI Contact Information</gco:CharacterString>
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                    <gco:CharacterString>Information for contacts at NCEI.</gco:CharacterString>
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                            <gmd:URL>https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords</gmd:URL>
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            <gmx:Anchor xlink:href="https://cmr.earthdata.nasa.gov/kms/concept/a4202721-0cba-4fa1-853f-890f146b04f9" xlink:actuate="onRequest">OCEAN &gt; ATLANTIC OCEAN &gt; NORTH ATLANTIC OCEAN</gmx:Anchor>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="place">place</gmd:MD_KeywordTypeCode>
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Units: n/a;
Observation Category: laboratory analysis;
Sampling Instrument: n/a</gco:CharacterString>
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Units: Fv/Fm;
Observation Category: laboratory analysis;
Sampling Instrument: Imaging PAM M-series chlorophyll fluorometer;
Sampling and Analyzing Method: Pulse amplitude modulated (PAM) measurements will be collected on treatment groups as previously described (May et al., 2020).  Briefly, sediment was removed from corals as described above and fragments were placed in a custom Teflon stand in ASW in a clean glass crystallizing dish, 1-2 h before the end of the dark cycle.  Lighting was provided by green headlamps to minimize any chlorophyll fluorescence excitation effects.  Data were collected on the top of the OFAV fragments.  During data analysis, a single circular area of interest was selected near the center of each fragment image prior to tissue wounding. After wounding, the area of interest for the top of the fragments was placed adjacent to, but not overlapping, the wound. Replicate fragments were evaluated as previously reported (Ralph et al., 2005).;
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Units: Dinoflagellates/mm2;
Observation Category: laboratory analysis;
Sampling Instrument: Adobe Photoshop, ImageJ;
Sampling and Analyzing Method: A sub-fragment will be removed at the termination of the experiment and fixed in  zinc-formalin (Z-fix).  Samples were fully decalcified using 0.5 EDTA and tissues were dehydrated using a series of ethanol dilutions prior to embedding in paraffin.  Paraffin blocks were sectioned at a 4 µm thickness and stained with hematoxylin and eosin (H&amp;E).  Photographs of 10 fields were randomly captured throughout the polyp surface body wall.  The area of the gastrodermis was measured and total algal symbiont density was calculated using the ImageJ cell counter plugin (RRID:SCR 003070). The number of algal cells was divided by the area of the gastroderm in each of the 10 images.  The average density was calculated for each tissue sample.;
Data Quality Method: Significant differences in Zoox Counts were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met.</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Orbicella faveolata Tissue Regeneration (calculated);
Units: Percent Regeneration;
Observation Category: laboratory analysis;
Sampling Instrument: Olympus MVX10 research macro zoom (0.63x) microscope equipped with a DP71 digital camera;  ImageJ software;
Sampling and Analyzing Method: Wounding of Orbicella faveolata fragments occurred 21 days prior to the 90 day experiment termination and photodocumented daily. Image analysis were used to determine the percent healing and healing rate of each fragment. Wound healing measurements were conducted using a metric ruler level with the wound and is included in each photograph to normalize any shifts in distance that may affect the magnification of the wound. The imaging software translated the distance between mm marks on the ruler into pixels to conduct the image analysis and measurements of changes in the area and perimeter of the wound.
Skeletal area for each coral image was determined using ImageJ (Schneider et al., 2012) with a modified macro from the NOAA/NCCOS Coral Disease and Health Consortium website (https://cdhc.noaa.gov/education/coral_assay.aspx; macro is provided as Appendix III).  Total area of bare skeleton and wound perimeter values were recorded for each fragment at time 0 and each experimental time point.;
Data Quality Method: Significant differences in Tissue Regeneration were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met.  A mixed-model was used to test for effects over time.</gco:CharacterString>
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Units: gram;
Observation Category: laboratory analysis;
Sampling Instrument: Analytical Balance;
Sampling and Analyzing Method: Buoyant (wet) weight is converted to dry weight based on seawater density derived from knowing the temperature, salinity and using aragonite density of 2.93 g cm-3.;
Data Quality Method: Significant differences in Dry Weight over the course of the experiment were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met.  A mixed-model was used to test for effects over time.
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                <gco:CharacterString>Parameter or Variable: Orbicella faveolata Buoyant Weight (measured);
Units: grams;
Observation Category: laboratory analysis;
Sampling Instrument: Analytical Balance;
Sampling and Analyzing Method: Net growth of Orbicella faveolata coral fragments is determined based on the buoyant weight technique of Jokiel et al. (1978). Each coral fragment is buoyantly weighed every two weeks using an analytical balance equipped with an underhook.;
Data Quality Method: Significant differences in Buoyant Weight over the course of the experiment were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met.  A mixed-model was used to test for effects over time.
Significant differences in Final Gain in Buoyant Weight were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met.</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Orbicella faveolata Total Protein (measured);
Units: grams;
Observation Category: laboratory analysis;
Sampling Instrument: Plate Reader;
Sampling and Analyzing Method: Total soluble proteins were isolated using a modified Comen’s method.  Proteins were quantified using a Pierce bicinchoninic acid assay on a Plate Reader;
Data Quality Method: Significant differences in Total Proteins were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met.</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Orbicella faveolata Total Lipids (measured);
Units: grams;
Observation Category: laboratory analysis;
Sampling Instrument: Analytical Balance;
Sampling and Analyzing Method: Total lipids were isolated using a modified Comen’s method.  Post-isolation, lipids were dried in a pre-weighed vial and the mass of the dried lipids was calculated by subtracting the initial weight of the vial from the final weight of the vial containing the dried lipids.;
Data Quality Method: Significant differences in Total Lipids were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met.</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Sediment Mean Particle Size (measured);
Units: microgram;
Observation Category: laboratory analysis;
Sampling Instrument: Malvern Mastersizer 3000;
Sampling and Analyzing Method: Turbidity water samples of each treatment were collected, centrifuged, and dried down.  The samples were run on a Malvern Mastersizer 3000 to determine particle size distribution of each treatment sample.;
Data Quality Method: Significant differences in Particle Size were determined using a nonparametric (Kruskal-Wallis) test with Dunn’s posttest.</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
          <gmd:organisationName>
            <gco:CharacterString>NOAA National Centers for Environmental Information</gco:CharacterString>
          </gmd:organisationName>
          <gmd:role>
            <gmd:CI_RoleCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="custodian">custodian</gmd:CI_RoleCode>
          </gmd:role>
        </gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation id="AMS">
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be92">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>camera</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>camera</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>An instrument for recording images.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_bee2">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>fluorometer</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>fluorometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>fluorometer</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_befe">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>laboratory analysis</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>laboratory analysis</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>use as an observation type</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be32">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>microscope</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>microscope</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>instrument is used in lab analyses

A microscope is an instrument used to see objects that are too small for the naked eye.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
    </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>