{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Katy E Klymus", "hasEmail": "mailto:kklymus@usgs.gov"}, "description": "The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P9BIGOS5", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5f85c8b682cebef40f14c5d0.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_5f85c8b682cebef40f14c5d0", "keyword": ["CERC molecular lab", "Clinch River", "Tennesee", "USGS:5f85c8b682cebef40f14c5d0", "Wallen's Bend", "assay optimization", "biota", "eDNA", "freshwater mussels", "qPCR"], "modified": "2020-11-19T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-83.0126, 36.579282, -83.004042, 36.583719", "theme": ["geospatial"], "title": "Mucket eDNA detection in Wallen\u2019s Bend, Clinch River, Tennessee, September 2019"}