{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Muruleedhara Byappanahalli", "hasEmail": "mailto:byappan@usgs.gov"}, "description": "Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method).  Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard exceedances for MF and qPCR methods; for enterococci: 70 colony-forming units (MF) and 1,000 calibrator cell equivalents and for E. coli: 235 colony-forming units (MF); currently, there are no established RWQS based for qPCR (i.e., CCE) results. \nThe CultureBasedData data include E. coli and enterococci culture-based bacterial counts. Data from the qpcr_v3 file includes results from quantitative polymerase chain reaction (qPCR) method for estimation of E. coli and enterococci counts by DNA-based methods. The Lat_Long file includes information regarding sampling sites at each of the four locations and their corresponding latitude and longitudes.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/F77P8XND", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5a4cf831e4b0d05ee8c4cae0.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_5a4cf831e4b0d05ee8c4cae0", "keyword": ["Lake Michigan", "Sleeping Bear Dunes National Lakeshore", "USGS:5a4cf831e4b0d05ee8c4cae0", "bacteria", "biota", "freshwater ecosystems", "polymerase chain reaction", "water quality", "water sampling"], "modified": "2025-01-24T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-86.2077, 44.6857, -86.0374, 44.8086", "theme": ["geospatial"], "title": "Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan."}