{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          Prostanoids are known to participate in the process of fibrogenesis. Because lung fibroblasts produce prostanoids and are believed to play a central role in the pathogenesis of idiopathic pulmonary fibrosis (IPF), we hypothesized that fibroblasts (HF) cultured from the lungs of patients with IPF (HF-IPF) have an altered balance between profibrotic (thromboxane [TX]A2) and antifibrotic (prostacyclin [PGI2]) prostaglandins (PGs) when compared with normal human lung fibroblasts (HF-NL).\n        \n        \n          Methods\n          We measured inducible cyclooxygenase (COX)-2 gene and protein expression, and a profile of prostanoids at baseline and after IL-1\u03b2 stimulation.\n        \n        \n          Results\n          In both HF-IPF and HF-NL COX-2 expression was undetectable at baseline, but was significantly upregulated by IL-1\u03b2. PGE2 was the predominant COX product in IL-1\u03b2-stimulated cells with no significant difference between HF-IPF and HF-NL (28.35 [9.09\u201389.09] vs. 17.12 [8.58\u201329.33] ng/106 cells/30 min, respectively; P = 0.25). TXB2 (the stable metabolite of TXA2) production was significantly higher in IL-1\u03b2-stimulated HF-IPF compared to HF-NL (1.92 [1.27\u20132.57] vs. 0.61 [0.21\u20131.64] ng/106 cells/30 min, respectively; P = 0.007) and the ratio of PGI2 (as measured by its stable metabolite 6-keto-PGF1\u03b1) to TXB2 was significantly lower at baseline in HF-IPF (0.08 [0.04\u20130.52] vs. 0.12 [0.11\u20130.89] in HF-NL; P = 0.028) and with IL-1\u03b2 stimulation (0.24 [0.05\u20131.53] vs. 1.08 [0.51\u20133.79] in HF-NL; P = 0.09).\n        \n        \n          Conclusion\n          An alteration in the balance of profibrotic and antifibrotic PGs in HF-IPF may play a role in the pathogeneses of IPF.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC107846/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/vgf4-j4ub", "issued": "2025-07-14", "keyword": ["cox-2-expression", "idiopathic-pulmonary-fibrosis", "lung-fibroblasts", "nih", "prostanoid-production"], "landingPage": "https://healthdata.gov/d/vgf4-j4ub", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis"}