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        <gco:CharacterString>This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification on hatch size and larval growth of walleye pollock (Theragra chalcogramma). Rising atmospheric concentrations of CO2 are predicted to decrease the pH of high-latitude oceans by 0.3-0.5 units by 2100. Because of their limited capacity for ion exchange, embryos and larvae of marine fishes are predicted to be more sensitive to elevated CO2 than juveniles and adults. Eggs and larvae of walleye pollock (Theragra chalcogramma) were incubated across a broad range of CO2 levels (280-2100 matm) to evaluate sensitivity in this critical resource species. Slightly elevated CO2 levels (450 matm) resulted in earlier hatching times, but differences among egg batches were greater than those observed across CO2 treatments. Egg batches differed significantly in size-at-hatch metrics, but we observed no consistent effect of CO2 level. In three independent experiments, walleye pollock were reared at ambient and elevated CO2 levels through the early larval stage (to 30 days post-hatch). Across trials, there were only minor effects of CO2 level on size and growth rate, but fish in the ambient treatments tended to be slightly smaller than fish reared at elevated CO2 levels. These results suggest that growth potential of early life stages of walleye pollock is resilient with respect to the direct physiological effects of ocean acidification.</gco:CharacterString>
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          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/datatype/details/5" xlink:actuate="onRequest">total alkalinity</gmx:Anchor>
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          <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="theme">theme</gmd:MD_KeywordTypeCode>
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                <gco:CharacterString>NODC DATA TYPES THESAURUS</gco:CharacterString>
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            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/198" xlink:actuate="onRequest">coulometer for DIC measurement</gmx:Anchor>
          </gmd:keyword>
          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/221" xlink:actuate="onRequest">microscope</gmx:Anchor>
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          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/199" xlink:actuate="onRequest">titrator</gmx:Anchor>
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              <gmd:title>
                <gco:CharacterString>NODC INSTRUMENT TYPES THESAURUS</gco:CharacterString>
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            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/obstype/details/2" xlink:actuate="onRequest">biological</gmx:Anchor>
          </gmd:keyword>
          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/obstype/details/62" xlink:actuate="onRequest">laboratory experiments</gmx:Anchor>
          </gmd:keyword>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="theme">theme</gmd:MD_KeywordTypeCode>
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            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1205" xlink:actuate="onRequest">NOAA Alaska Fisheries Science Center</gmx:Anchor>
          </gmd:keyword>
          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/976" xlink:actuate="onRequest">NOAA Pacific Marine Environmental Laboratory</gmx:Anchor>
          </gmd:keyword>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="dataCentre">dataCentre</gmd:MD_KeywordTypeCode>
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            <gmd:CI_Citation>
              <gmd:title>
                <gco:CharacterString>NODC COLLECTING INSTITUTION NAMES THESAURUS</gco:CharacterString>
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            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1205" xlink:actuate="onRequest">NOAA Alaska Fisheries Science Center</gmx:Anchor>
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          <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="dataCentre">dataCentre</gmd:MD_KeywordTypeCode>
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                <gco:CharacterString>NODC SUBMITTING INSTITUTION NAMES THESAURUS</gco:CharacterString>
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            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/project/details/653" xlink:actuate="onRequest">NODC Ocean Acidification Scientific Data Stewardship (OADS)</gmx:Anchor>
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            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/project/details/675" xlink:actuate="onRequest">US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)</gmx:Anchor>
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                <gco:CharacterString>Additional metadata files were received or created for this dataset. These updates were copied into the about/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession.</gco:CharacterString>
              </gmd:rationale>
              <gmd:dateTime>
                <gco:DateTime>2015-02-11T15:24:10Z</gco:DateTime>
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                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>NCEI Accession 0125007 v1.2</gco:CharacterString>
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                                <gmd:CI_OnlineResource>
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                                    <gmd:URL>https://www.ncei.noaa.gov/archive/accession/0125007/1.2</gmd:URL>
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                                    <gco:CharacterString>NCEI Accession 0125007 v1.2</gco:CharacterString>
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                                    <gco:CharacterString>published 2015-02-11T15:24:10Z</gco:CharacterString>
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  <gmd:dataQualityInfo>
    <gmd:DQ_DataQuality>
      <gmd:scope>
        <gmd:DQ_Scope>
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            <gmd:MD_ScopeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ScopeCode" codeListValue="nonGeographicDataset">nonGeographicDataset</gmd:MD_ScopeCode>
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        <gmd:LI_Lineage>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Dissolved Inorganic Carbon;
Abbreviation: DIC;
Unit: umol/kg;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Siphoning tube;
Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity) coupled to a UIC 5014 coulometer.;
Preservation method: saturated HgCl2;
Preservative correction: no correction.;
Uncertainty: less than 0.05%;
Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.;
Researcher name: Jeremy T. Mathis;
Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Total alkalinity;
Abbreviation: TA;
Unit: umol/kg;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Siphoning tube;
Analyzing instrument: Alkalinity titrator;
Preservation method: saturated HgCl2;
Preservative correction: no correction.;
Uncertainty: less than 0.05%;
Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.;
Researcher name: Jeremy T. Mathis;
Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: pH;
Abbreviation: pH;
pH scale: Seawater;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Calculated;
Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity;
At what temperature was pH reported: 8.0;
Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.;
Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827;
Researcher name: Jeremy T. Mathis;
Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: pCO2 (fCO2) autonomous;
Abbreviation: pCO2;
Unit: uatm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Calculated;
Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity;
At what temperature was pCO2 reported: 8.0;
Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.;
Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827;
Researcher name: Jeremy T. Mathis;
Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Calcite saturation state;
Abbreviation: CAsat;
Unit: omega;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: calculated;
Detailed sampling and analyzing information: calculated using CO2sys program;
Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.;
Researcher name: Jeremy T. Mathis;
Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Aragonite saturation state;
Abbreviation: ARsat;
Unit: omega;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: calculated;
Detailed sampling and analyzing information: calculated using CO2sys program;
Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.;
Researcher name: Jeremy T. Mathis;
Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Mean standard length;
Abbreviation: SLmean;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: measured;
Sampling instrument: dissecting microscope;
Analyzing instrument: ImagePro image analysis system;
Detailed sampling and analyzing information: Newly hatched larvae were sampled and measured daily throughout the hatching period. Each day during the hatch cycle, up to 20 newly hatched larvae from each beaker were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.;
Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date;
Quality flag convention: "sizeDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Mean body height;
Abbreviation: HTmean;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: measured;
Sampling instrument: dissecting microscope;
Analyzing instrument: ImagePro image analysis system;
Detailed sampling and analyzing information: Newly hatched larvae were sampled and measured daily throughout the hatching period. Each day during the hatch cycle, up to 20 newly hatched larvae from each beaker were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.;
Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date;
Quality flag convention: "sizeDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Mean eye diameter;
Abbreviation: IDmean;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: measured;
Sampling instrument: dissecting microscope;
Analyzing instrument: ImagePro image analysis system;
Detailed sampling and analyzing information: Newly hatched larvae were sampled and measured daily throughout the hatching period. Each day during the hatch cycle, up to 20 newly hatched larvae from each beaker were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.;
Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date;
Quality flag convention: "sizeDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Mean yolk area;
Abbreviation: YAmean;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: measured;
Sampling instrument: dissecting microscope;
Analyzing instrument: ImagePro image analysis system;
Detailed sampling and analyzing information: Newly hatched larvae were sampled and measured daily throughout the hatching period. Each day during the hatch cycle, up to 20 newly hatched larvae from each beaker were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.;
Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date;
Quality flag convention: "sizeDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Mean body condition;
Abbreviation: Kmean;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: calculated;
Sampling instrument: dissecting microscope;
Analyzing instrument: ImagePro image analysis system;
Detailed sampling and analyzing information: Newly hatched larvae were sampled and measured daily throughout the hatching period. Each day during the hatch cycle, up to 20 newly hatched larvae from each beaker were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.;
Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date;
Quality flag convention: "sizeDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Mean time to hatch;
Abbreviation: DPFmean;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: measured;
Detailed sampling and analyzing information: Newly hatched larvae were sampled daily throughout the hatching period.;
Quality flag convention: "hatchDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Number of fish hatching from incubation beaker;
Abbreviation: hatch_n;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Detailed sampling and analyzing information: Newly hatched larvae were sampled daily throughout the hatching period.;
Quality flag convention: "hatchDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.;
Biological subject: walleye pollock;
Species ID: Gadus chalcogrammus (renamed from Theragra chalcogramma, 2014);
Researcher name: Thomas P. Hurst;
Researcher institution: NOAA Alaska Fisheries Science Center</gco:CharacterString>
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                <gco:CharacterString>NOAA National Centers for Environmental Information</gco:CharacterString>
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                <gco:CharacterString>NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.</gco:CharacterString>
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              <gmd:dateTime>
                <gco:DateTime>2015-04-22T00:00:00</gco:DateTime>
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      <gmd:maintenanceNote>
        <gco:CharacterString>Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.</gco:CharacterString>
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            <gco:CharacterString>NOAA National Centers for Environmental Information</gco:CharacterString>
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            <gmd:CI_RoleCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="custodian">custodian</gmd:CI_RoleCode>
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  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation id="AMS">
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be29">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>coulometer for DIC measurement</gco:CharacterString>
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            <gco:CharacterString>coulometer for DIC measurement</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>measures dissolved inorganic carbon (DIC)

A known amount of seawater is dispensed into a stripping chamber where it is acidified and purged with an inert gas. The amount of CO2 in the resulting gas stream is determined by absorbing the CO2 in an absorbent. The pH of the solution is monitored by measuring the transmittance .</gco:CharacterString>
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            <gco:CharacterString>microscope</gco:CharacterString>
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            <gco:CharacterString>instrument is used in lab analyses

A microscope is an instrument used to see objects that are too small for the naked eye.</gco:CharacterString>
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