{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["005:18"], "contactPoint": {"fn": "Naoumkina, Marina", "hasEmail": "mailto:marina.naoumkina@usda.gov"}, "description": "<p dir=\"ltr\">This dataset contains RNA\u2011seq count tables, sample metadata, analysis scripts, and computational environment files used to characterize transcriptional variation in a somaclonal mutant of <i>Gossypium hirsutum</i> (upland cotton) that exhibits impaired chloroplast photochemistry. The mutant originated during tissue culture and segregates into positive, negative, and mutant (Tsens) phenotypic classes. RNA\u2011seq was performed on leaf tissue collected under optimal greenhouse conditions and under drought and heat stress to identify gene expression changes associated with the mutant phenotype and to characterize stress\u2011responsive transcriptional programs in upland cotton. The dataset includes featureCounts output, metadata tables, the full R analysis script, and session information documenting all software versions. Raw sequencing reads are available under NCBI BioProject <b>PRJNA1445904</b>.</p>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63389169", "format": "txt", "mediaType": "text/plain", "title": "sessionInfo.txt"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63389172", "format": "R", "mediaType": "text/plain", "title": "limma_leaf.R"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63389175", "format": "csv", "mediaType": "text/csv", "title": "design_stress.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63389181", "format": "csv", "mediaType": "text/csv", "title": "featurecounts_drought_heat.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63389184", "format": "csv", "mediaType": "text/csv", "title": "featurecounts_T3s_Leaves.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63389187", "format": "csv", "mediaType": "text/csv", "title": "gene_map.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63417462", "format": "csv", "mediaType": "text/csv", "title": "edf.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/63590664", "format": "md", "mediaType": "text/plain", "title": "README.md"}], "identifier": "10.15482/USDA.ADC/31920066.v1", "keyword": ["Gossypium hirssutum", "Photosynthensis", "drought", "heat", "mutant", "source code"], "license": "https://creativecommons.org/publicdomain/zero/1.0/", "modified": "2026-04-17", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "temporal": "2024-09-01/2024-12-30", "title": "A defect in photochemistry underlies reduced biomass in a cotton (<i>Gossypium hirsutum</i> L.) mutant"}