{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Andrew Ghio", "hasEmail": "mailto:ghio.andy@epa.gov"}, "description": "Figure 2.  Cell non-heme iron concentrations following 4 (A) and 24 (B) hr exposures of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA).  Incubations were without and with 200 \u03bcM FAC.  Exposures to CB-Air, CB-O3, and SRFA did not impact cell non-heme iron relative to media at either time point.  Exposure to FAC increased cell non-heme iron but co-exposure with CB-O3 was associated with significantly increased non-heme iron concentrations at both 4 (A) and 24 (B) hr.  Exposures of BEAS-2B cells to FAC increased cell ferritin levels after 24 hr relative to media alone (C).  However, co-exposure of CB-O3, and SRFA with FAC was associated with significantly elevated cell ferritin concentrations (C).  Two-way ANOVA indicated that both addition of FAC and exposure group significantly impacted cell non-heme iron.  \n\nFigure 3.  Release of IL-8 and IL-6 after 24 hr exposure of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA).  Exposure to CB-O3 and SRFA significantly increased the release of IL-8 and IL-6. FAC treatment significantly diminished this response. \n\nThis dataset is associated with the following publication:\nGhio, A., D.H. Gonzalez, S.E.P. Paulson, J. Soukup, L. Dailey, M. Madden, B. Mahler, S.A. Elmore, M.C. Schladweiler, and U. Kodavanti. Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect.   TOXICOLOGIC PATHOLOGY. Society of Toxicology, RESTON, VA,  48(7): 887-898, (2020).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1519298/ozone%20and%20hulis%20figure%202A%20sci%20hub.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "ozone and hulis figure 2A sci hub.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1519298/ozone%20and%20hulis%20figure%202b%20sci%20hub.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "ozone and hulis figure 2b sci hub.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1519298/ozone%20and%20hulis%20figure%202c%20sci%20hub.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "ozone and hulis figure 2c sci hub.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1519298/ozone%20and%20hulis%20figure%203A%20sci%20hub.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "ozone and hulis figure 3A sci hub.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1519298/ozone%20and%20hulis%20figure%203B%20sci%20hub.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "ozone and hulis figure 3B sci hub.xlsx"}], "identifier": "https://doi.org/10.23719/1519298", "keyword": ["Ozone", "Rats", "air pollution", "carbon black", "fulvic acid", "fulvic acid-like substance", "inflammation", "iron", "lung diseases"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2020-01-01", "programCode": ["020:000"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1177/0192623320961017", "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%202A%20sci%20hub.xlsx", "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%202b%20sci%20hub.xlsx", "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%202c%20sci%20hub.xlsx", "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%203A%20sci%20hub.xlsx", "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%203B%20sci%20hub.xlsx"], "rights": null, "title": "Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect"}