gov.noaa.nodc:0227133 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2025-05-16T15:46:37Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Shotgun proteomics of thermally challenged Caribbean reef corals (NCEI Accession 0227133) 2021-07-30 publication 2021-03-22 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0227133 NCEI Archive Management System 0227133 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Anderson B. Mayfield NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) andersonblairmayfield@gmail.com https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information resourceProvider NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information resourceProvider Anderson Mayfield Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) anderson.mayfield@noaa.gov https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information principalInvestigator Mayfield, Anderson Cooperative Institute for Marine and Atmospheric Studies anderson.mayfield@noaa.gov https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information author NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information contributor tableDigital A proteome profiling approach was taken to characterize the cellular biology of massive corals of the Upper Florida Keys exposed to experimentally elevated temperatures. Specifically, Orbicella faveolata specimens from two inshore (Cheeca Rocks & The Rocks) and one offshore reef (Little Conch) were incubated at either 33°C for 5 days or 32°C days for 31 days (with controls maintained at 30°C), and proteins were extracted from a subset of 16 samples (representing multiple coral genotypes at each temperature x time), separated by liquid chromatography, and sequenced by mass spectrometry (MS). The associated, open-access manuscript (Mayfield et al. 2021) includes all methodological details needed to interpret the proteomic data. However, I have nevertheless reiterated the majority of these details in this document to aid those in navigating the NCEI data submission. Alongside this detailed methodological treatise, I have also uploaded 16 RAW files generated by the MS (Q Exactive™, Thermo-Fisher Scientific [TFS]), 16 MZML (open-access MS mass peaks) files, 16 MZID (open-access MS results) files, two sequence libraries needed to query the proteomic data (fasta files for the coral host & its dinoflagellate [family Symbiodiniaceae] endosymbionts), and a tab-delimited “online supplemental data file” (OSDF) that 1) describes which samples were analyzed; 2) defines MS jargon and explains how to interpret the MS output; and 3) presents all data in a distilled format that is more amenable to analysis by traditional statistical approaches. Please note that there are 32 MZID files, rather than 16, because each of the 16 MZML files was separately queried against each of the two aforementioned fasta sequence libraries. These data were collected to better understand how reef-building corals respond to elevated temperatures at the cellular level. Funding Agency: National Oceanic and Atmospheric Administration completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0227133 Preview graphic PNG 0227133 NCEI ACCESSION NUMBER 2020-12-22 publication biological data CORAL ORGANIC CHEMICALS theme NODC DATA TYPES THESAURUS chromatograph mass spectrometer instrument NODC INSTRUMENT TYPES THESAURUS biological laboratory analyses theme NODC OBSERVATION TYPES THESAURUS NOAA Atlantic Oceanographic and Meteorological Laboratory dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Atlantic Oceanographic and Meteorological Laboratory dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Coastal Waters of Florida Florida Keys National Marine Sanctuary North Atlantic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/OAR/AOML > Atlantic Oceanographic and Meteorological Laboratory, OAR, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Numeric Data Sets > Biology theme CoRIS Discovery Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Proteomic Analysis EARTH SCIENCE > Biosphere > Zoology > Corals > Scleractinia (stony corals) EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs EARTH SCIENCE > Oceans > Marine Biology > Coral theme CoRIS Theme Thesaurus EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY EARTH SCIENCE > SOLID EARTH > GEOMORPHIC LANDFORMS/PROCESSES > COASTAL LANDFORMS > CORAL REEFS theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian GAS CHROMATOGRAPHS > GAS CHROMATOGRAPHS MASS SPECTROMETERS > MASS SPECTROMETERS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Liquid chromatography instrument Provider Instruments M/V GBN1 platform Provider Platforms COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Cheeca Rocks (24N080W0009) COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Conch Reef (24N080W0011) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Cheeca Rocks (24N080W0009) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Conch Reef (24N080W0011) place CoRIS Place Thesaurus OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF AMERICA OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Little Conch The Rocks place Provider Place Names DKGAXF Send2NCEI Submission Package ID accessLevel: Public otherRestrictions Cite as: Mayfield, Anderson (2021). Shotgun proteomics of thermally challenged Caribbean reef corals (NCEI Accession 0227133). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0227133. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Mayfield, A. B., Aguilar, C., Kolodziej, G., Enochs, I. C., & Manzello, D. P. (2021). Shotgun Proteomic Analysis of Thermally Challenged Reef Corals. Frontiers in Marine Science, 8. https://doi.org/10.3389/fmars.2021.660153 2021-07-19 publication International DOI Foundation (IDF) https://doi.org/10.3389/fmars.2021.660153 https://doi.org/10.3389/fmars.2021.660153 HTTPS https://doi.org/10.3389/fmars.2021.660153 related journal article information Mayfield, Anderson B. author Aguilar, Catalina author Kolodziej, Graham author Enochs, Ian C. author Manzello, Derek P. author Frontiers Media SA publisher documentDigital Front. Mar. Sci. 8 ISSN 2296-7745 crossReference sciencePaper eng; USA utf8 environment oceans biota -80.61573 -80.50205 24.89742 24.95375 2017-07-15 2017-08-31 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. XLSX DOCX FASTA MZID MZML RAW 26737.348 https://www.ncei.noaa.gov/archive/accession/0227133 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/227133 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/227133 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0184/0227133/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0227133 v1.1 was published. 2021-07-30T21:07:44Z NCEI Accession 0227133 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0227133/1.1 HTTPS NCEI Accession 0227133 v1.1 published 2021-07-30T21:07:44Z download dataset Parameter or Variable: protein concentrations (measured); Units: presence/absence (0/1); Observation Category: laboratory analysis; Sampling Instrument: SCUBA diver; Sampling and Analyzing Method: A "shotgun" proteomics approach was taken in which proteins were purified, digested, separated (by nano-liquid chromatography), and sequenced (by mass spectrometry). Proteins were scored as either being present (1) or absent (0) from a sample.; Data Quality Method: A detailed treatise on quality control has been provided in the document "Mayfield et al. supplemental material." Briefly and non-exhaustively, we filtered out proteins found only in 1-2 of all samples. asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian chromatograph chromatograph Gas, ion, liquid or other chromatograph mass spectrometer mass spectrometer Instrument that measure the massess and relative concentrations of atoms and molecules