{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "accrualPeriodicity": "irregular",
  "bureauCode": [
    "026:00"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "GeneLab Outreach",
    "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
  },
  "description": "The general objective of the study was to determine modulation of gene expression by environmental factors with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment which we like to call relative microgravity referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf one batch was left at 3g (3g) one batch was returned to 1g (3g>1g) while a third batch was returned to 1g but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times each time with 4 batches of 60 larvae.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "description": "GeneLab Study Page",
      "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-60",
      "format": "HTML",
      "mediaType": "text/html",
      "title": "Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization"
    }
  ],
  "identifier": "nasa_genelab_GLDS-60_gkqw-6gd9",
  "issued": "2018-06-26",
  "keyword": [
    "array-scanning-and-feature-extraction-protocol",
    "growth-protocol",
    "hypergravity",
    "normalization-data-transformation-protocol",
    "nucleic-acid-extraction-protocol",
    "nucleic-acid-hybridization-to-array-protocol",
    "nucleic-acid-labeling-protocol",
    "p-mtab-43163",
    "p-mtab-43164",
    "p-mtab-43165",
    "p-mtab-43166",
    "p-mtab-43167",
    "p-mtab-43168",
    "p-mtab-43169",
    "treatment-protocol"
  ],
  "landingPage": "https://data.nasa.gov/dataset/exposure-of-zebrafish-larvae-to-relative-microgravity-between-5-6-days-post-fertilization",
  "modified": "2025-04-23",
  "programCode": [
    "026:005"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Aeronautics and Space Administration"
  },
  "theme": [
    "Earth Science"
  ],
  "title": "Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization"
}