{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "John Kenneke", "hasEmail": "mailto:kenneke.john@epa.gov"}, "description": "The instructions for analysis are described in Kolli et al. (2019) along with a sample Illumina sequence file available with a ready-to-run VirtualMachine that can also be accessed at http://toxicology.uga.edu/resources/dna_methylation_analysis/. Supporting data for sequencing and qPCR raw data are available at https://doi.org/10.5281/zenodo.8313356, sharing qPCR raw data files for the endpoint for male and female fish from CFX Manger (Ver. 3.0), qPCR quantification data, TGBS raw sequences, and a TGBS metadata file with sample information. \n\nThis dataset is associated with the following publication:\nKolli, R., T. Glenn, R. Bringolf,, W. Henderson, B. Cummings, and J. Kenneke. Changes in CpG Methylation of the Vitellogenin 1 Promoter in Adult Male Zebrafish after Exposure to 17\u03b1-Ethynylestradiol.   ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 43(7): 1547-1556, (2024).", "distribution": [{"accessURL": "https://doi.org/10.5281/zenodo.8313356", "title": "https://doi.org/10.5281/zenodo.8313356"}, {"accessURL": "https://toxicology.uga.edu/resources/dna_methylation_analysis/", "title": "https://toxicology.uga.edu/resources/dna_methylation_analysis/"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1531458/Supplementary.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "Supplementary.docx"}], "identifier": "https://doi.org/10.23719/1531458", "keyword": ["DNA methylation", "ecotoxicology", "endocrine-disrupting compounds", "estrogenic compounds", "vitellogenin", "zebrafish"], "license": "https://pasteur.epa.gov/license/sciencehub-license-non-epa-generated.html", "modified": "2024-03-28", "programCode": ["020:000"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1002/etc.5879"], "rights": null, "title": "Changes in CpG Methylation of the Vitellogenin 1 Promoter in Adult Male Zebrafish after Exposure to 17\u03b1-Ethynylestradiol"}