{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          Animal and organ culture experiments have shown beneficial inhibitory estrogen effects on post injury neointima development. The purpose of this study was to investigate whether such estrogen effects are influenced by the estrogen receptor antagonist ICI 182,780. Different concentrations of 17\u03b2-estradiol and the phytoestrogens genistein and daidzein were tested.\n        \n        \n          Methods\n          F emale New Zealand White rabbits were benumbed. In situ vascular injury of the thoracic and abdominal aorta was performed by a 3F Fogarty catheter. Segments of 5 mm were randomised and held in culture for 21 days. Three test series were performed: 1) control group \u2013 20 \u03bcM ICI \u2013 30 \u03bcM ICI \u2013 40 \u03bcM ICI. 2) control group \u2013 20 \u03bcM ICI \u2013 40 \u03bcM 17\u03b2-estradiol \u2013 40 \u03bcM 17\u03b2-estradiol + 20 \u03bcM ICI. 3) control group \u2013 20 \u03bcM ICI \u2013 40 \u03bcM daidzein \u2013 40 \u03bcM daidzein + 20 \u03bcM ICI \u2013 20 \u03bcM genistein \u2013 20 \u03bcM genistein + 20 \u03bcM ICI. After 21 days the neointima-media-ratio was evaluated.\n        \n        \n          Results\n          1) Treatment with ICI 182,780 did not reduce neointima formation significantly (p = 0.05). 2) 40 \u03bcM 17\u03b2-estradiol alone (p < 0.0001) and in combination with 20 \u03bcM ICI (p < 0.0001) reduced neointima formation significantly. 3) 20 \u03bcM genistein alone (p = 0.0083) and combined with 20 \u03bcM ICI (p = 0.0053) reduced neointima formation significantly. 40 \u03bcM daidzein did not have a significant (p = 0.0637) effect.\n        \n        \n          Conclusions\n          The estrogen receptor antagonist ICI 182,780 did not modulate the inhibitory estrogen effects on post injury neointima formation. These results do not support the idea that such effects are mediated by vascular estrogen receptors.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC119852/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/myiw-tpfu", "issued": "2025-07-14", "keyword": ["estradiol-treatment", "neointima-formation", "nih", "phytoestrogen-effects", "vascular-injury"], "landingPage": "https://healthdata.gov/d/myiw-tpfu", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Reduction of post injury neointima formation due to 17\u03b2-estradiol and phytoestrogen treatment is not influenced by the pure synthetic estrogen receptor antagonist ICI 182,780 in vitro"}