{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Sharma, Manan", "hasEmail": "mailto:manan.sharma@usda.gov"}, "description": "<p>Biological soil amendments are an essential input in organic lettuce production. However, these BSAs can introduce or transfer pathogens like <em>Escherichia coli</em> O157:H7 to lettuce in pre-harvest environments. This study evaluated the effect of BSAs on survival of non-pathogenic and pathogenic <em>E. coli</em> in soils and transfer to lettuce. Romaine lettuce was grown with controlled light, temperature, and relative humidity. Soil was amended (side-dressed) with either heat treated poultry pellets (HTPP), HTPP with corn steep liquor (CSL), seabird guano (SBG), SBG with CSL, or left unamended (UA). Soils were co-inoculated with non-pathogenic, rifampicin-resistant <em>E. coli</em> TVS 353 and two chloramphenicol-resistant <em>E. coli</em> O157:H7 isolates (100 mL of 10<sup>6</sup> CFU/mL). <em>E. coli</em> survival over 28 days was evaluated. On day 28, Romaine lettuce was harvested, and presence of <em>E. coli</em> was determined.</p>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/49136773", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Ecoli_levels_recovered_from_amended_soil.xlsx"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/49136776", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Soil_Temperature&Moisture.xlsx"}], "identifier": "10.15482/USDA.ADC/26999374.v1", "keyword": ["Bacterial survival studies", "E. coli O157: H7", "biological soil amendments", "fresh produce contamination", "produce safety"], "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2025-06-30", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "temporal": "2023-08-21/2023-09-18", "title": "Data from: Biological Soil Amendments Can Support Survival of Pathogenic and Non-Pathogenic Escherichia coli in Soils and Sporadic Transfer to Romaine Lettuce"}