{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          A number of transcription factors coordinate differentiation by simultaneously regulating gene expression and cell proliferation. CCAAT/enhancer binding protein alpha (C/EBP\u03b1) is a basic/leucine zipper transcription factor that integrates transcription with proliferation to regulate the differentiation of tissues involved in energy balance. In the pituitary, C/EBP\u03b1 regulates the transcription of a key metabolic regulator, growth hormone.\n        \n        \n          Results\n          We examined the consequences of C/EBP\u03b1 expression on proliferation of the transformed, mouse GHFT1-5 pituitary progenitor cell line. In contrast to mature pituitary cells, GHFT1-5 cells do not contain C/EBP\u03b1. Ectopic expression of C/EBP\u03b1 in the progenitor cells resulted in prolongation of both growth 1 (G1) and the DNA synthesis (S) phases of the cell cycle. Transcription activation domain 1 and 2 of C/EBP\u03b1 were required for prolongation of G1, but not of S. Some transcriptionally inactive derivatives of C/EBP\u03b1 remained competent for G1 and S phase prolongation. C/EBP\u03b1 deleted of its leucine zipper dimerization functions was as effective as full-length C/EBP\u03b1 in prolonging G1 and S.\n        \n        \n          Conclusion\n          We found that C/EBP\u03b1 utilizes mechanistically distinct activities to prolong the cell cycle in G1 and S in pituitary progenitor cells. G1 and S phase prolongation did not require that C/EBP\u03b1 remained transcriptionally active or retained the ability to dimerize via the leucine zipper. G1, but not S, arrest required a domain overlapping with C/EBP\u03b1 transcription activation functions 1 and 2. Separation of mechanisms governing proliferation and transcription permits C/EBP\u03b1 to regulate gene expression independently of its effects on proliferation.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC101385/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/m4ek-rv53", "issued": "2025-07-14", "keyword": ["cebp-alpha", "cell-cycle-regulation", "growth-hormone", "nih", "pituitary-cells"], "landingPage": "https://healthdata.gov/d/m4ek-rv53", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "CCAAT/Enhancer Binding Protein alpha uses distinct domains to prolong pituitary cells in the Growth 1 and DNA Synthesis phases of the cell cycle"}