{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Christian Andersen", "hasEmail": "mailto:andersen.christian@epa.gov"}, "description": "The intergenerational impact of engineered nanomaterials in plants is a key knowledge gap in the literature. A soil microcosm study was performed to assess the effects of multi-generational exposure of wheat (Triticum aestivum L.) to cerium oxide nanoparticles (CeO2-NPs). Seeds from plants that were exposed to 0, 125, and 500 mg CeO2-NPs/kg soil (Ce-0, Ce-125 or Ce-500, respectively) in first generation (S1) were cultivated in factorial combinations of Ce-0, Ce-125 or Ce-500 to produce second generation (S2) plants. The factorial combinations for first/second generation treatments in Ce-125 were S1-Ce-0/S2-Ce-0, S1-Ce-0/S2-Ce-125, S1-Ce-125/S2-Ce-0 and S1-Ce-125/S2-Ce-125, and in Ce-500 were S1-Ce-0/S2-Ce-0, S1-Ce-0/S2-Ce-500, S1-Ce-500/S2-Ce-0 and S1-Ce-500/S2-Ce-500. Agronomic, elemental, isotopic, and synchrotron X-ray fluorescence (XRF) and X-ray absorption near-edge spectroscopy (XANES) data were collected on second generation plants. Results showed that plants treated during the first generation only with either Ce-125 or Ce-500 (e.g. S1-Ce-125/S2-Ce-0 or S1-Ce-500/S2-Ce-0) had reduced accumulation of Ce (61 or 50%), Fe (49 or 58%) and Mn (34 or 41%) in roots, and \u03b415N (11 or 8%) in grains compared to the plants not treated in both generations (i.e. S1-Ce-0/S2-Ce-0). Plants treated in both generations with Ce-125 (i.e. S1-Ce-125/S2-Ce-125) produced grains that had lower Mn, Ca, K, Mg and P relative to plants treated in the second generation only (i.e. S1-Ce-0/S2-Ce-125). In addition, synchrotron XRF elemental chemistry maps of soil/plant thin-sections revealed limited transformation of CeO2-NPs with no evidence of plant uptake or accumulation. The findings demonstrated that first generation exposure of wheat to CeO2-NPs affects the physiology and nutrient profile of the second generation plants. However, the lack of concentration-dependent responses indicate that complex physiological processes are involved which alter uptake and metabolism of CeO2-NPs in wheat. \n\nThis dataset is associated with the following publication:\nRico, C., M. Johnson, M. Marcus, and C. Andersen. Intergenerational responses of wheat (Triticum aestivum L.) to cerium oxide nanoparticles exposure.   Environmental Science: Nano. RSC Publishing, Cambridge,  UK, 4: 700-711, (2017).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1502592/CeO2%20Wheat%20Intergenerational%20Data%20Repository%20for%208-24-18.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "CeO2 Wheat Intergenerational Data Repository for 8-24-18.xlsx"}], "identifier": "https://doi.org/10.23719/1502592", "keyword": ["ammonium nitrate", "engineered nanomaterials (ENMs)", "intergenerational effects", "isotope", "isotopic discrimination", "nitrogen"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2018-08-27", "programCode": ["020:095"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1039/c7en00057j"], "rights": null, "title": "Intergenerational responses of wheat to CeO2 nanoparticles, growth and nutrient contents"}