{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Main, Dorrie", "hasEmail": "mailto:dorrie@wsu.edu"}, "description": "<p>Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:</p>\n<ul>\n<li>\n<p>oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,</p>\n</li>\n<li>\n<p>PCR product size,</p>\n</li>\n<li>\n<p>positional constraints within the source (template) sequence, and</p>\n</li>\n<li>\n<p>possibilities for ectopic priming (amplifying the wrong sequence)</p>\n</li>\n<li>\n<p>many other constraints.</p>\n</li>\n</ul>\n<p>All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.</p>\n<div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Website Pointer for CottonGen Primer3.</p> <p>File Name: Web Page, url: <a href=\"https://www.cottongen.org/tools/primer3\" target=\"_blank\">https://www.cottongen.org/tools/primer3</a> </p></li></ul>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://www.cottongen.org/tools/primer3", "mediaType": "text/html", "title": "https://www.cottongen.org/tools/primer3"}], "identifier": "10113/AA23039", "keyword": ["ARS", "CottonGen", "NP301", "Online database", "Primer3", "data.gov"], "license": "https://www.usa.gov/publicdomain/label/1.0/", "modified": "2024-02-13", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "title": "CottonGen Primer3"}