{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "accrualPeriodicity": "irregular",
  "bureauCode": [
    "026:00"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "GeneLab Outreach",
    "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
  },
  "description": "The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "description": "GeneLab Study Page",
      "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-76",
      "format": "HTML",
      "mediaType": "text/html",
      "title": "Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day"
    }
  ],
  "identifier": "nasa_genelab_GLDS-76_6g6v-gqya",
  "issued": "2021-05-21",
  "keyword": [
    "array-scanning-and-feature-extraction",
    "growth-protocol",
    "labeling",
    "normalization-data-transformation",
    "nucleic-acid-hybridization",
    "p-mtab-48667",
    "p-mtab-48668",
    "p-mtab-48669",
    "p-mtab-48670",
    "p-mtab-48671",
    "p-mtab-48672",
    "p-mtab-48673",
    "rna-extraction",
    "stress",
    "treatment-protocol"
  ],
  "landingPage": "https://data.nasa.gov/dataset/zebrafish-larvae-submitted-to-simulated-microgravity-on-a-clinostat-clino-at-5dpf-for-one-",
  "license": "http://www.usa.gov/publicdomain/label/1.0/",
  "modified": "2025-04-23",
  "programCode": [
    "026:005"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Aeronautics and Space Administration"
  },
  "theme": [
    "Earth Science"
  ],
  "title": "Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day"
}