gov.noaa.nodc:0265154 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-07-23T07:50:39Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Cellular abundances of bacterioplankton and eukaryotic picoplankton measured by flow cytometry in water samples collected on NOAA Ship Ronald H. Brown during the West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) in the northern California current ecosystem from 2016-05-24 to 2016-06-16 (NCEI Accession 0265154) 2022-10-18 publication 2022-10-11 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0265154 NCEI Archive Management System 0265154 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Linda D. Rhodes NOAA Northwest Fisheries Science Center (NMFS/NWFSC) linda.rhodes@noaa.gov http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information resourceProvider NOAA Northwest Fisheries Science Center (NMFS/NWFSC) http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information resourceProvider Linda D. Rhodes NOAA Northwest Fisheries Science Center (NMFS/NWFSC) linda.rhodes@noaa.gov http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information principalInvestigator Nicolaus G. Adams NOAA Northwest Fisheries Science Center (NMFS/NWFSC) Nicolaus.Adams@noaa.gov http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information principalInvestigator Simone R. Alin NOAA Pacific Marine Environmental Laboratory (PMEL) simone.r.alin@noaa.gov https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information collaborator Richard Feely NOAA Pacific Marine Environmental Laboratory (PMEL) Richard.A.Feely@noaa.gov https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information collaborator Rhodes, Linda D. NOAA Northwest Fisheries Science Center http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information author Adams, Nicolaus G. NOAA Northwest Fisheries Science Center Nicolaus.Adams@noaa.gov http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information author Alin, Simone R. NOAA Pacific Marine Environmental Laboratory https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Feely, Richard A. NOAA Pacific Marine Environmental Laboratory https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author NOAA Northwest Fisheries Science Center (NMFS/NWFSC) http://www.nwfsc.noaa.gov/index.cfm HTTP NOAA Northwest Fisheries Science Center (NMFS/NWFSC) website Institution web page information contributor NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information contributor tableDigital Changes in ocean conditions such as chemical and thermal shifts are habitat pressures on marine organisms. As the basis of the marine food web, microorganisms such as bacteria and phytoplankton are responsible for important biogeochemical processes, such as nutrient cycling, and the primary production. They are also part of the environment surrounding aquatic organisms such as marine mammals, and serve as a source of both beneficial and harmful microbes associated with larger organisms. Due to their small size and direct physiological interactions with seawater, these microorganisms can rapidly respond to environmental changes, resulting in shifts in community composition and in relative abundances of community members. Shifts at these trophic levels can cause a ripple effect in the structure and function of the ecosystem for coastal and offshore species. Most assessments of biological responses to ocean acidification (OA) have been performed by controlled conditions, and there have been few opportunities to evaluate real-world relationships between biology and OA. This project leverages the sampling and chemical analyses conducted during the northern leg of the 2016 West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) to document ocean chemistry in the California Current during a period of likely coastal upwelling and greater ocean acidification. During that cruise, water from the same water samples that were used for chemical measurements by PMEL were preserved & analyzed for cellular abundances of bacterioplankton and eukaryotic picoplankton. Determine relationship(s) of microbial abundances to ocean chemistry conditions Funding Information: NOAA Ocean Acidification Program (Microbial relationships to ocean acidification chemistry in the northern California current ecosystem, OAP/NRDD project number 21405) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0265154 Preview graphic PNG 0265154 NCEI ACCESSION NUMBER 2022-09-29 publication bacteria BACTERIA - BACTERIAL DENSITY CELL COUNTS diatom MICROBIOLOGY MICROPLANKTON PHYTOPLANKTON theme NODC DATA TYPES THESAURUS Flow Cytometer instrument NODC INSTRUMENT TYPES THESAURUS laboratory analyses theme NODC OBSERVATION TYPES THESAURUS NOAA Ship Ronald H. Brown platform NODC PLATFORM NAMES THESAURUS NOAA Northwest Fisheries Science Center NOAA Pacific Marine Environmental Laboratory dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Northwest Fisheries Science Center dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) project NODC PROJECT NAMES THESAURUS Coastal Waters of Southeast Alaska and British Columbia Columbia River estuary - Washington/Oregon North Pacific Ocean Olympic Coast National Marine Sanctuary place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/NMFS/NWFSC > Northwest Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce DOC/NOAA/OAR/PMEL > Pacific Marine Environmental Laboratory, OAR, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian WCOA2016 project Cruise ID 33RO20160505 project EXPOCODE Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords Lines 8 - 17 project Reference Section ID EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE > DIATOMS EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Cryptophytes Prochlorococcus Synechococcus picophytoplankton theme Provider Keywords Bacteria_total_cell_per_ml Big_diatoms_cell_per_ml Cryptophytes_cell_per_ml Euk_phytoplankton_cell_per_ml Prochlorococcus_cell_per_ml Synechococcus_cell_per_ml theme Provider Variable Abbreviations FLOW CYTOMETRY instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ships platform Global Change Master Directory (GCMD) Platform Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ronald H. Brown (call sign: WTEC, ICES code: 33RO, 1997-) platform ICES/SeaDataNet Ship Codes OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian West Coast of CONUS and British Columbia (Canada), partial place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Rhodes, Linda D.; Adams, Nicolaus G.; Alin, Simone R.; Feely, Richard A. (2022). Cellular abundances of bacterioplankton and eukaryotic picoplankton measured by flow cytometry in water samples collected on NOAA Ship Ronald H. Brown during the West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) in the northern California current ecosystem from 2016-05-24 to 2016-06-16 (NCEI Accession 0265154). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0265154. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Feely, R. A., Alin, S. R., Carter, B., Bednaršek, N., Hales, B., Chan, F., Hill, T. M., Gaylord, B., Sanford, E., Byrne, R. H., Sabine, C. L., Greeley, D., & Juranek, L. (2016). Chemical and biological impacts of ocean acidification along the west coast of North America. Estuarine, Coastal and Shelf Science, 183, 260–270. https://doi.org/10.1016/j.ecss.2016.08.043 2016-12 publication International DOI Foundation (IDF) https://doi.org/10.1016/j.ecss.2016.08.043 https://doi.org/10.1016/j.ecss.2016.08.043 HTTPS https://doi.org/10.1016/j.ecss.2016.08.043 journal article information Feely, Richard A. Pacific Marine Environmental Laboratory/NOAA https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Alin, Simone R. author Carter, Brendan author Bednaršek, Nina author Hales, Burke author Chan, Francis author Hill, Tessa M. author Gaylord, Brian author Sanford, Eric author Byrne, Robert H. author Sabine, Christopher L. author Greeley, Dana author Juranek, Lauren author Elsevier BV publisher documentDigital Estuarine, Coastal and Shelf Science 183 ISSN 0272-7714, pp. 260-270 crossReference project NOAA West Coast Ocean Acidification Cruises (WCOA) 2022 publication https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/WCOA.html HTTPS https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/WCOA.html Related resource information NOAA National Centers for Environmental Information (NCEI) publisher documentDigital crossReference project NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program eng; USA utf8 environment oceans biota -129.4498 -123.9122 40.2463 52.3992 2016-05-24 2016-06-16 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Excel 0.316 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0265154.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/0265154 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/265154 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/265154 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0208/0265154/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0265154 v1.1 was published. 2022-10-18T20:33:18Z NCEI Accession 0265154 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0265154/1.1 HTTPS NCEI Accession 0265154 v1.1 published 2022-10-18T20:33:18Z download dataset Parameter or Variable: Synechococcus abundance; Abbreviation: Synechococcus_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Synechococcus; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes Parameter or Variable: Eukarotic picophytoplankton abundance; Abbreviation: Euk_phytoplankton_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Prymnesiophyceae, Chrysophyceae, Cryptophyceae, Pelagophyceae, Pinguiophyceae,Chlorarachniophyceae, Eustigmatophyceae, Pavlovophyceae, Trebouxiophyceae, Prasnophyceae; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes Parameter or Variable: Large diatom (non-chain-forming) abundance; Abbreviation: Big_diatoms_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Bacillariophyceae; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes Parameter or Variable: Prochlorococcus abundance; Abbreviation: Prochlorococcus_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Prochlorococcus; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes Parameter or Variable: Cryptophyte abundance; Abbreviation: Cryptophytes_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Cryptophyceae; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes Parameter or Variable: Total bacteria and archaea abundance; Abbreviation: Bacteria_total_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Cellular double-stranded nucleic acid stained with SYBR Green. Sample gating based on forward scatter, side scatter and green fluorescence (515 - 545 nm). Data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: D. K. Button, B. R. Robertson. Use of High-Resolution Flow Cytometry to Determine the Activity and Distribution of Aquatic Bacteria (Chapter 21). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Bacteria, Archaea; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian Flow Cytometer Flow Cytometer A method to enumerate bacterial and phytoplankton cells in seawater and estimate their spherical diameter Flow cytometers are laboratory based instruments used to measure cells and particles from aqueous samples. The instrument was initially developed for analysis of Human blood cells for medical purposes, but this technology was adopted by the ocean science community by several investigators, principally Penny Chischolm out of the Massachusetts Institute of Technology, in late 20th century. Since then, the technique has been broadly applied, and it is a unique measurement of microbial populations in the ocean because it provides one at a time measures of cells, to extrapolate features such as fluorescence and cell size. Flow cytometers have been adapted for a number of oceanographic applications such as ship board measurement, and more precise measurement of planktonic fractions. Additionally, the flow cytometer has been adapted for in-situ deployment (see the corresponding nodc instrument code). NOAA Ship Ronald H. Brown Updated information from ICES for UNITED STATES platform Ronald H. Brown Flag : UNITED STATES - US (ISO Country Code) Call Sign : WTEC Title : NOAA Ship Platform Class : Research vessel IMO : 9105786 Pennant : R 104 Commissioned Date : 1997-07-19 Current Length : 83.5 Built : 1997 Information Source : http://www.moc.noaa.gov/rb/index.html (URL) Lloyds URL : http://www.seasearcher.com/lmiu/vessels/overview.htm?vesselID=277516 *General Comments:* Name RESEARCHER II planned during build was never used.