{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          Recent evidence suggests that estrogens exert effects in different tissues throughout the body, and that the estrogen receptor \u03b2 (ER\u03b2) may be important for the action of estrogen (17-\u03b2-estradiol) on the skeleton. The cellular localization of ER\u03b2 in the human intervertebral disc, however, has not yet been explored.\n        \n        \n          Methods\n          Human disc tissue and cultured human disc cells were used for immunocytochemical localization of ER\u03b2. mRNA was isolated from cultured human disc cells, and RT-PCR amplification of ER\u03b2 was employed to document molecular expression of this receptor. Cultured human disc cells were tested to determine if 17-\u03b2-estradiol stimulated cell proliferation.\n        \n        \n          Results\n          In this report data are presented which provide evidence for ER\u03b2 gene expression in human intervertebral disc cells in vivo and in vitro. Culture of annulus cells in the presence of 10-7 M 17-\u03b2-estradiol significantly increased cell proliferation.\n        \n        \n          Conclusions\n          These data provide new insight into the biology of cells in the annulus of the intervertebral disc.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC65546/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/cyji-rb6h", "issued": "2025-07-14", "keyword": ["17-beta-estradiol", "annulus-cells", "estrogen-receptor-beta", "intervertebral-disc", "nih"], "landingPage": "https://healthdata.gov/d/cyji-rb6h", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Expression and localization of estrogen receptor-\u03b2 in annulus cells of the human intervertebral disc and the mitogenic effect of 17-\u03b2-estradiol in vitro"}