{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "accrualPeriodicity": "irregular",
  "bureauCode": [
    "026:00"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "GeneLab Outreach",
    "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
  },
  "description": "Genome-wide detection of novel non-coding RNAs in S. cerevisiae by modulating an RNase P pathway through the depletion of a component RPP1. Nearly 400,000 36-mer oligonucleotide probes tiling the entire yeast genome including the mitochondrial chromosome with an average gap of 10 bases between two consecutive probes were synthesized on glass slides using a mask-less array synthesizer. RNA samples for hybridizing to the arrays were extracted from a conditional lethal allele of S. cerevisiae created by placing the RPP1 gene under control of GAL10 promoter. It allowed the expression of RPP1 in galactose-containing culture medium but suppressed its expression in glucose-containing medium. A wild-type isogenic strain was used as a control. Both strains were initially grown in galactose-containing medium and subsequently transferred and resuspended into glucose-containing medium. Eight arrays were hybridized with RNA extracted from the Rpp1-depleted cells at 0 4 7 12 16 21 30h and the control cell at 30h after initial transfer to glucose-containing medium.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "description": "GeneLab Study Page",
      "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-10",
      "format": "HTML",
      "mediaType": "text/html",
      "title": "Global Identification of Noncoding RNAs in S. cerevisiae"
    }
  ],
  "identifier": "nasa_genelab_GLDS-10",
  "issued": "2018-06-26",
  "keyword": [
    "bioassay_data_transformation",
    "data-collection",
    "data-transformation",
    "feature_extraction",
    "labeling",
    "normalization-data-transformation",
    "nucleic-acid-hybridization",
    "p-gse4275-1",
    "p-gse4275-2",
    "p-gse4275-3",
    "rna-extraction",
    "timepoint"
  ],
  "landingPage": "https://data.nasa.gov/dataset/global-identification-of-noncoding-rnas-in-s-cerevisiae",
  "modified": "2025-04-23",
  "programCode": [
    "026:005"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Aeronautics and Space Administration"
  },
  "theme": [
    "Earth Science"
  ],
  "title": "Global Identification of Noncoding RNAs in S. cerevisiae"
}