{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "McKenzie Smith", "hasEmail": "mailto:mksmith@usgs.gov"}, "description": "These data describe delivery of novel gene silencing agents (GSAs) to leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i> using cell penetrating peptides (CPPs).  These CPPs were tested for their ability to infiltrate <i>P. australis</i> leaf tissue using confocal microscopy. In addition, a series of novel gene silencing agents targeting a putative phytoene desaturase (<i>PDS</i>) gene were developed including twelve antisense oligonucleotides (ASOs), five artificial microRNAs (amiRNAs), and amiRNA-expressing plasmids and a cassette. GSAs were applied to living <i>P. australis</i> plants in conjunction with cell penetrating peptides and an infiltration medium at multiple charge ratios and application frequencies. Reduced expression of <i>PDS</i> was then characterized using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Confocal microscopy and qPCR results  are available herein.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P13ETPDI", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.66b78f5dd34eebcf8bb3da9f.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_66b78f5dd34eebcf8bb3da9f", "keyword": ["Phragmites australis", "RNA interference", "USGS:66b78f5dd34eebcf8bb3da9f", "farming", "gene expression", "gene silencing", "invasive species", "molecular biology"], "modified": "2025-01-28T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-90.87453, 32.29091, -90.85238, 32.31296", "theme": ["geospatial"], "title": "Quantitative polymerase chain reaction and confocal microscopy data describing cell penetrating peptide-mediated delivery of gene silencing agents to the invasive common reed Phragmites australis"}