{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Eric Villegas", "hasEmail": "mailto:villegas.eric@epa.gov"}, "description": "Cryptosporidiosis, giardiasis, and microsporidiosis are important waterborne diseases. In the\nstandard for wastewater treatment plant (WWTP) effluents in China and other countries, fecal\ncoliform is the only microbial indicator, raising concerns about the potential for pathogen\ntransmission through WWPT effluent reuse. In this study, we collected 50 effluent samples\n(30 L/sample) from three municipal WWTPs in Shanghai, China and analyzed for Cryptosporidium\nspp., Giardia duodenalis and Enterocytozoon bieneusi by microscopy and/or PCR. Moreover,\npropidium monoazide (PMA)-PCR was used to assess the viability of oocysts/cysts. The microscopy\nand PCR-positive rates for Cryptosporidium spp. were 62% and 40%, respectively. The occurrence\nrates of G. duodenalis were 96% by microscopy and 92\u2013100% by PCR analysis of three genetic loci.\nFurthermore, E. bieneusi was detected in 70% (35/50) of samples by PCR. Altogether, ten\nCryptosporidium species or genotypes, two G. duodenalis genotypes, and 11 E. bieneusi genotypes\nwere found, most of which were human-pathogenic. The chlorine dioxide disinfection employed in\nWWTP1 and WWTP3 failed to inactivate the residual pathogens; 93% of the samples from WWTP1 and\n83% from WWTP3 did not meet the national standard on fecal coliform levels. Thus, urban WWTP\neffluents often contain residual waterborne human pathogens. \n\nThis dataset is associated with the following publication:\nMa, J., Y. Feng, Y. Hu, E. Villegas , and L. Xiao. Human infective potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in urban wastewater treatment plant effluents.   JOURNAL OF WATER AND HEALTH. IWA Publishing, London,  UK, 14(4): 411-423, (2016).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/295/NO%20EPA%20DATASETS%20WERE%20GENERATED%20IN%20THIS%20STUDY.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "NO EPA DATASETS WERE GENERATED IN THIS STUDY.docx"}], "identifier": "A-dfnd-295", "keyword": ["cryptosporidium", "giardia", "microsporidia", "occurrence", "wastewater", "wastewater treatment plant", "water reuse"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2016-09-21", "programCode": ["020:096"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["http://jwh.iwaponline.com/content/early/2016/01/04/wh.2016.192"], "rights": null, "title": "Human infective potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in urban wastewater treatment plant effluents"}