These are results of a series of laboratory experiments to determine if topical application of methoprene and 20-ecdysone can terminate reproductive diapause of the weevil, Ceratapion basicorne, which is a recently permitted biological control agent of yellow starthistle (Centaurea solstitialis). Adult weevils feed on leaves, creating pin holes, and lay eggs inside leaves. Diapausing weevils were treated with various doses of methoprene (0, 0.01, 0.1, 1.0 micrograms) dissolved in acetone in experiments 1 and 2. They were treated sequentially first with acetone or 20-ecdysone (1.0 microgram) and then with methoprene (1.0 microgram) in experiment 3 and were treated with 20-ecdysone followed by methoprene in experiment 4.
Resource Title: data dictionary.
File Name: JH Data Dictionary.csv
Resource Description: description of data fields
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/microsoft-365/excel
Resource Title: experiment 1.
File Name: JH expt1 data.csv
Resource Description: Methoprene dissolved in acetone was applied topically at doses of 0.0, 0.01 and 0.1 and 1.0 \u03bcg per female weevil, and the number of feeding holes and eggs were recorded daily on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21\u00b0C).
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/microsoft-365/excel
Resource Title: experiment 2.
File Name: JH expt2 data.csv
Resource Description: Methoprene dissolved in acetone was applied topically at doses of 0.0 and 1.0 \u03bcg to female weevils that did not produce eggs in experiment 1. The number of feeding holes and eggs were recorded daily on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21\u00b0C).
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/microsoft-365/excel
Resource Title: experiment 3.
File Name: JH expt3 data.csv
Resource Description: Three types of treatments were applied with sequential applications 2 days apart: 1) acetone + acetone [AA: control], 2) acetone + methoprene [AM], and 20-ecdysone + methoprene 174 [2M]. All doses were 1.0 \u03bcg. The number of feeding holes and eggs were recorded every 2 days on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21\u00b0C).
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/microsoft-365/excel
Resource Title: experiment 4.
File Name: JH expt4 data.csv
Resource Description: Females from experiment 3 that did not oviposit consistently were treated with 1.0 \u03bcg of 20-ecdysone followed 2 days later by 1.0 \u03bcg of methoprene. The treatments AA, AM, 2M refer to experiment 3. The number of feeding holes and eggs were recorded every 2 days on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21\u00b0C).
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/microsoft-365/excel