{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Narrowe, Adrienne", "hasEmail": "mailto:adrienne.narrowe@usda.gov"}, "description": "<p>A 24-h in vitro culturing method was used to determine whether fructooligosaccharide (FOS) treatment effects on the human gut microbiome has a differential effect by age, using fecal samples  from 18 adults in 3 age groups with 6 individuals for each group, young adult (25\u201335 years of age), adult (36\u201350 years of age), and older adult (51\u201370 years of age). Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883714: <a href=\"https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714\">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714</a>), total bacterial qPCR, Bifidobacterium genus specific q-PCR, and short chain fatty acid concentrations. </p>\n<p>Resources in this dataset:</p>\n<ul>\n<li>\n<p>Resource Title: Sample metadata\nFile Name: FOS_sample_attributes.csv</p>\n</li>\n<li>\n<p>Resource Title: Short Chain Fatty Acid concentrations\nFile Name: FOS_SCFA_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Total bacterial and Bifidobacterium genus qPCR\nFile Name: total_bacteria_and_Bifidobacterium_specific_qPCR.csv</p>\n</li>\n</ul>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/44540789", "format": "csv", "mediaType": "application/csv", "title": "FOS_sample_attributes_0.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/44540792", "format": "csv", "mediaType": "text/plain", "title": "FOS_SCFA_data_0.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/44540795", "format": "csv", "mediaType": "application/csv", "title": "total_bacteria_and_Bifidobacterium_specific_qPCR_0.csv"}], "identifier": "10.15482/USDA.ADC/1529151", "keyword": ["16S rRNA sequence data", "ARS", "NP306", "bifidobacteria", "data.gov", "fructooligosaccharides", "prebiotics", "short chain fatty acids"], "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2025-11-21", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "spatial": "{\"type\": \"Point\", \"coordinates\": [-75.187092456102, 40.078126249741]}", "temporal": "2021-05-01/2021-05-01", "title": "Data from: Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner"}