gov.noaa.nodc:0216077 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2025-05-16T15:50:32Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077) 2020-09-09 publication 2020-08-04 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0216077 NCEI Archive Management System 0216077 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Anderson B. Mayfield NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) andersonblairmayfield@gmail.com https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information resourceProvider NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information resourceProvider Anderson Mayfield University of Miami (UM) abm64@miami.edu http://www.miami.edu/ HTTP University of Miami (UM) website Institution web page information principalInvestigator Mayfield, Anderson B. University of Miami (UM) anderson.mayfield@noaa.gov http://www.miami.edu/ HTTP University of Miami (UM) website Institution web page information author Cooperative Institute for Marine and Atmospheric Studies, University of Miami https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information author Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information author University of Miami (UM) http://www.miami.edu/ HTTP University of Miami (UM) website Institution web page information contributor tableDigital In this work, I took a protein profiling approach known as iTRAQ approach to characterize and quantify the proteins synthesized by 12 experimental coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu"; 21°56’18.01’’N, 120°44’45.54’’E) and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan"; 22°01'23.30"N, 120°41'18.29"E) and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML (open-access mass spectrometry data) files (SHVTS-A.mzml & SHVTS-B.mzml), two MZID files (SHVTS-A.mzid & SHVTS-B.mzid), and a tab-delimited file that describes which samples were labeled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome described in Mayfield et al. (2016 Mol Ecol) and found here: http://symbiont.iis.sinica.edu.tw/s_hystrix/static/html/#stat. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific). These data were collected to better understand the cellular biology of thermo-tolerant corals (i.e., those that can tolerate wide fluctuations in temperature). For more details, please see the articles referenced in the data package and on my personal website: coralreefdiagnostics.com. Funding Agency: Friendly Bear Editorial Service Funding Agency: MacArthur Foundation completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0216077 Preview graphic PNG 0216077 NCEI ACCESSION NUMBER 2020-08-01 publication CORAL ORGANIC CHEMICALS theme NODC DATA TYPES THESAURUS mass spectrometer swimmer/diver instrument NODC INSTRUMENT TYPES THESAURUS biological laboratory analyses theme NODC OBSERVATION TYPES THESAURUS University of Miami dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Atlantic Oceanographic and Meteorological Laboratory dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS South China Sea (Nan Hai) Taiwan Strait or Formosa Strait (T'ai-Wan Hai-Hsia) place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/OAR/AOML > Atlantic Oceanographic and Meteorological Laboratory, OAR, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Numeric Data Sets > Biology theme CoRIS Discovery Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Proteomic Analysis EARTH SCIENCE > Biosphere > Zoology > Corals > Scleractinia (stony corals) EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs EARTH SCIENCE > Oceans > Marine Biology > Coral theme CoRIS Theme Thesaurus EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY EARTH SCIENCE > SOLID EARTH > GEOMORPHIC LANDFORMS/PROCESSES > COASTAL LANDFORMS > CORAL REEFS theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian MASS SPECTROMETERS > MASS SPECTROMETERS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian COUNTRY/TERRITORY > Taiwan > Hengchung > Nanwan Bay (21N120E0008) OCEAN BASIN > Pacific Ocean > Hengchung > Nanwan Bay (21N120E0008) place CoRIS Place Thesaurus OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN OCEAN > PACIFIC OCEAN > WESTERN PACIFIC OCEAN > SOUTH CHINA SEA place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian BELJ6L Send2NCEI Submission Package ID accessLevel: public otherRestrictions Cite as: Mayfield, Anderson B.; Cooperative Institute for Marine and Atmospheric Studies, University of Miami; Atlantic Oceanographic and Meteorological Laboratory (AOML) (2020). Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0216077. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Proteomic signatures of corals from thermodynamic reefs 2020 publication https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=558adcff310b48279b6692dbe3ea41c0 HTTPS Proteomic signatures of corals from thermodynamic reefs MassIVE Metadata Record information crossReference Mayfield, A., Chan, P.-H., Putnam, H. M., Chen, C.-S., & Fan, T.-Y. (2012). The effects of a variable temperature regime on the physiology of the reef-building coralSeriatopora hystrix: results from a laboratory-based reciprocal transplant. Journal of Experimental Biology. https://doi.org/10.1242/jeb.071688 2012-01-01 publication International DOI Foundation (IDF) https://doi.org/10.1242/jeb.071688 https://doi.org/10.1242/jeb.071688 HTTPS https://doi.org/10.1242/jeb.071688 related journal article information Mayfield, Anderson National Museum of Marine Biology and Aquarium; author Chan, Pei-Hsun National Museum of Marine Biology and Aquarium; author Putnam, Hollie M Hawaii Institute of Marine Biology author Chen, Chii-Shiarng National Museum of Marine Biology and Aquarium; author Fan, Tung-Yung National Museum of Marine Biology and Aquarium; author The Company of Biologists publisher documentDigital Journal of Experimental Biology ISSN 1477-9145, ISSN 0022-0949 crossReference Mayfield, A. B. (2020). Proteomic Signatures of Corals from Thermodynamic Reefs. Microorganisms, 8(8), 1171. https://doi.org/10.3390/microorganisms8081171 2020-08-01 publication International DOI Foundation (IDF) https://doi.org/10.3390/microorganisms8081171 https://doi.org/10.3390/microorganisms8081171 HTTPS https://doi.org/10.3390/microorganisms8081171 NOAA Central Library: Journal Article information Mayfield, Anderson B. Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration, 4301 Rickenbacker Causeway, Miami, FL 33149, USA author MDPI AG publisher documentDigital Microorganisms 8(8) ISSN 2076-2607, p. 1171 crossReference collection eng; USA utf8 environment oceans biota 120.68841 120.74598 21.93834 22.02314 2010-05-14 2010-06-15 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. XLSX ODS PDF MZID MZML RAW FA 1450.828 https://www.ncei.noaa.gov/archive/accession/0216077 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/216077 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/216077 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0159/0216077/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0216077 v1.1 was published. 2020-09-09T12:46:25Z NCEI Accession 0216077 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0216077/1.1 HTTPS NCEI Accession 0216077 v1.1 published 2020-09-09T12:46:25Z download dataset Parameter or Variable: protein concentrations (measured); Units: ratio to control (unitless); Observation Category: laboratory analysis; Sampling Instrument: manual sampling (SCUBA); Sampling and Analyzing Method: The isobaric tags for relative and absolute (protein) quantification (iTRAQ) approach of Ross et al. (2004 Mol Cell Proteomics) was used to label 12 experimental coral protein samples and two internal control samples that were used to normalize for batch-to-batch variation. Since there are only eight iTRAQ labels at present, this signifies that only eight proteins can be analyzed simultaneously. Given that 12 proteins were analyzed herein, two iTRAQ batches ("A" and "B" in the supplemental Excel file) were required to process all samples (6 experimental samples+1 normalizer/control sample in each batch).; Data Quality Method: An exhaustive series of quality control analyses were undertaken; please consult the attached methods file. Briefly, we only considered proteins sequenced in multiple batches that contained the iTRAQ tag asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian mass spectrometer mass spectrometer Instrument that measure the massess and relative concentrations of atoms and molecules swimmer/diver swimmer/diver not an instrument - use as observation type