{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Aaron W. Aunins", "hasEmail": "mailto:aaunins@usgs.gov"}, "description": "The Grand Calumet River flows through northwestern Indiana and was designated an EPA  Area of Concern (AOC) in 1987. There are multiple legacy pollutants in the river including but not limited to heavy metals and PCBs. To investigate potential causes and patterns of eutrophication in the river, water samples were collected for 16S rRNA microbial and eukaryotic 18S rRNA amplicon sequencing during April - September 2021. This data release describes the resulting sequencing data.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P93261E5", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.649b40a4d34ef77fcb02d50d.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_649b40a4d34ef77fcb02d50d", "keyword": ["DNA Sequencing", "Eukaryotic 18S metabarcoding", "Freshwater river metagenome", "Microbial 16S metabarcoding", "USGS:649b40a4d34ef77fcb02d50d", "biota", "environment"], "modified": "2026-06-23T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-87.7148, 41.4551, -87.1078, 41.7160", "theme": ["geospatial"], "title": "Grand Calumet River Area of Concern Metabarcoding Data"}