{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["006:55"], "contactPoint": {"fn": "Samuel P. Forry", "hasEmail": "mailto:samuel.forry@nist.gov"}, "description": "This repository includes the raw data, analysis code, and results of a systematic analysis of natural transformation (i.e., horizontal gene transfer; HGT) of bacteria in a naturally occurring microbiome (human stool) following exposure to free extracellular DNA (eDNA) that coded for antibiotic resistance. A full description of this effort is available in the associated publication.\u00a0", "distribution": [{"description": "Analysis code of ddPCR data.", "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/ddPCR/FIG1-ddPCR-Analysis.Rmd", "mediaType": "application/octet-stream", "title": "Figure 1 - ddPCR Analysis"}, {"description": "This READEME file describes the organization this data record.", "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/00README.txt.sha256", "mediaType": "text/plain", "title": "README"}, {"description": "This READEME file describes the organization this data record.", "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/CFU/FIG2-CFU-Analysis.Rmd", "mediaType": "application/octet-stream", "title": "Read Me"}, {"description": "This compressed folder contains the raw data collected in the analysis of aforementioned samples. This includes CFU count results, ddPCR gene quantification, long-read next-generation sequencing (Nanopore) of isolates, and shotgun metagenomic sequencing characterization of microbiome samples.", "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Data.zip", "mediaType": "application/zip", "title": "Data"}, {"description": "This file describes the organization of this data record.", "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/00README.txt", "mediaType": "text/plain", "title": "READEME file"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/CFU/FIG2-CFU-Analysis.Rmd.sha256", "mediaType": "text/plain", "title": "README"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG4-WGS-AnalyzingProcessedReads.Rmd", "mediaType": "application/octet-stream"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG4-WGS-AnalyzingProcessedReads.Rmd.sha256", "mediaType": "text/plain"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG5-WGS-AnalyzingProcessedReads.Rmd", "mediaType": "application/octet-stream"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG5-WGS-AnalyzingProcessedReads.Rmd.sha256", "mediaType": "text/plain"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/ddPCR/FIG1-ddPCR-Analysis.Rmd.sha256", "mediaType": "text/plain"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Data.zip.sha256", "mediaType": "text/plain"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/HGT_STORMS_Checklist.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet"}, {"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/HGT_STORMS_Checklist.xlsx.sha256", "mediaType": "text/plain"}], "identifier": "ark:/88434/mds2-3579", "issued": "2024-11-07", "keyword": ["antibiotic resistance", "horizontal gene transfer", "microbiome", "natural transformation"], "landingPage": "https://data.nist.gov/od/id/mds2-3579", "language": ["en"], "license": "https://www.nist.gov/open/license", "modified": "2024-09-26 00:00:00", "programCode": ["006:045"], "publisher": {"@type": "org:Organization", "name": "National Institute of Standards and Technology"}, "theme": ["Bioscience:Microbial measurements"], "title": "Measuring Microbial Community-Wide Antibiotic Resistance Propagation via Natural Transformation in the Human Gut Microbiome"}