gov.noaa.nodc:0302978 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-07-14T14:56:07Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) NCCOS Assessment: Use of ‘Omics to Support Coral Interventions: Coral fertilization trials data to Optimize Sexual Reproduction in corals from Key Largo, FL from 2022-08-13 to 2022-08-17, 2023-08-04 to 2023-08-05, and 2023-09-05 (NCEI Accession 0302978) 2025-04-04 publication 2025-03-28 creation International DOI Foundation (IDF) 10.25921/h4me-aw30 NOAA National Centers for Environmental Information gov.noaa.nodc:0302978 NCEI Archive Management System 0302978 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher NOAA National Centers for Coastal Ocean Science (NCCOS) https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information resourceProvider Cheryl M. Woodley NOAA National Centers for Coastal Ocean Science (NCCOS) Cheryl.woodley@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information principalInvestigator Richard Drake Medical University of South Carolina (MUSC) Draker@musc.edu principalInvestigator Cheryl M. Woodley NOAA National Centers for Coastal Ocean Science (NCCOS) Cheryl.woodley@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information pointOfContact NOAA National Centers for Coastal Ocean Science (NCCOS) NCCOS Data Manager nccos.data@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information pointOfContact Elizabeth McDonald NOAA National Centers for Coastal Ocean Science (NCCOS) elizabeth.mcdonald@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information collaborator Caroline Kittrell Medical University of South Carolina (MUSC) collaborator Grace Grimsley Medical University of South Carolina (MUSC) collaborator Emily Parsons College of Charleston http://www.cofc.edu/ HTTP College of Charleston website Institution web page information collaborator Courtney Saldaña Consolidated Safety Services-Dynamac (CSS) courtney.saldana@noaa.gov https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information collaborator Zachary Moffitt Consolidated Safety Services-Dynamac (CSS) zachary.moffitt@noaa.gov https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information collaborator Kimberly Burgess College of Charleston http://www.cofc.edu/ HTTP College of Charleston website Institution web page information collaborator Lisa May NOAA National Centers for Coastal Ocean Science (NCCOS) https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information collaborator Saunders, Janet NOAA National Centers for Coastal Ocean Science https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information author Videtto, Emily NOAA National Centers for Coastal Ocean Science https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information author Woodley, Cheryl M. NOAA National Centers for Coastal Ocean Science https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information author Young, Lyndsay Medical University of South Carolina (MUSC) author Drake, Richard Medical University of South Carolina (MUSC) author NOAA National Centers for Coastal Ocean Science (NCCOS) https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information contributor College of Charleston http://www.cofc.edu/ HTTP College of Charleston website Institution web page information contributor tableDigital This dataset is comprised of fertilization rates, glycan imaging data, and proteomics results to better understand gamete compatibility factors. The dataset includes gametes from three species, Acropora cervicornis, Acropora Palmata, Orbicella faveolata, which were collected in three locations near Key Largo, Florida (Ofav: Horseshoe Reef, Apal: North Dry Rocks, Acer: Carysfort) during three different time periods (August 2022, August 2023, and September 2023). Successful coral sexual reproduction is foundational to the achieving coral reef restoration, yet reproductive failure is a significant bottleneck to realizing restoration goals. The overall goal of this project is to identify factors that are involved in gamete compatibility and build on this knowledge to improve fertilization success and other downstream choke points in reproduction, such as larval settlement. The 2023 project was an extension of our previous work in genotype compatibility and kinship for Acropora cervicornis (Acer), Acropora palmata (Apal), and Orbicella faveolata (Ofav). The goals of this portion of the overall project were 1) in collaboration with the Drake Lab at the Medical University of South Carolina, we sought to identify glycans present in egg and sperm gametes across multiple genotypes and within three different coral species using Matrix Assisted Laser Desorption/Ionization (MALDI) imaging spectrometry, and 2) correlate the glycan expression with fertilization rates between egg and sperm gametes of the same species. Funding Agency: NOAA Coral Reef Conservation Program Funding Agency: NOAA National Centers for Coastal Ocean Science completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0302978 Preview graphic PNG 0302978 NCEI ACCESSION NUMBER 2025-03-04 publication biological data CORAL images theme NODC DATA TYPES THESAURUS camera laboratory analysis mass spectrometer instrument NODC INSTRUMENT TYPES THESAURUS biological laboratory analyses theme NODC OBSERVATION TYPES THESAURUS College of Charleston NOAA National Centers for Coastal Ocean Science dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA National Centers for Coastal Ocean Science dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Coral Reef Conservation Program (CRCP) CORAL REEF STUDIES project NODC PROJECT NAMES THESAURUS Florida Keys National Marine Sanctuary North Atlantic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication Coral Restoration Foundation Everglades National Park Florida Bay Interagency Science Center Mote Marine Laboratory dataCentre Contributing Data Centers DOC/NOAA/NOS/NCCOS > National Centers for Coastal Ocean Science, National Ocean Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian 31374 Use of 'Omics to Support Coral Interventions: Optimizing Sexual Reproduction project CRCP Project NOAA Coral Reef Conservation Program Project Titled: Use of 'Omics to Support Coral Interventions: Optimizing Sexual Reproduction, Project #31374 project Provider Project Names Genetic Data Sets > Proteomic Analysis Numeric Data Sets > Biology Visual Images > Corals theme CoRIS Discovery Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals EARTH SCIENCE > Biosphere > Zoology > Corals > ESA Listed Species EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Proteomic Analysis EARTH SCIENCE > Biosphere > Zoology > Corals > Reproduction > Sexual Reproduction > Spawning EARTH SCIENCE > Biosphere > Zoology > Corals > Scleractinia (stony corals) theme CoRIS Theme Thesaurus EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > SOLID EARTH > GEOMORPHIC LANDFORMS/PROCESSES > COASTAL LANDFORMS > CORAL REEFS theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NCCOS Research Data Type > Laboratory Experiment NCCOS Research Priority > Coastal Change: Vulnerability, Mitigation, and Restoration NCCOS Research Topic > Restoration theme NCCOS Keywords Acropora cervicornis Acropora palmata Coral Sexual Reproduction Gamete Compatibility Glycan Intensity Orbicella faveolata Peptide Intensity theme Provider Keywords CAMERA > CAMERA MASS SPECTROMETERS > MASS SPECTROMETERS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Orbitrap Fusion Lumos LC-MS/MS TIMS (2022) and Bruker Scimax (2023) instrument Provider Instruments COUNTRY/TERRITORY > United States of America > Florida > Dade County > Horseshoe Reef (25N080W0033) COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Carysfort Reef (25N080W0013) COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Dry Rocks (25N080W0011) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Biscayne Bay > Horseshoe Reef (25N080W0033) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Carysfort Reef (25N080W0013) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Dry Rocks (25N080W0011) place CoRIS Place Thesaurus OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF AMERICA OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NCCOS Research Locations > Region > Atlantic Ocean NCCOS Research Locations > U.S. States and Territories > Florida place NCCOS Location Keywords Carysfort Reef Florida Coast Horseshoe Reef Key Largo North Dry Rocks Reef place Provider Place Names 7MX6YY Send2NCEI Submission Package ID accessLevel: public otherRestrictions Cite as: Saunders, Janet; Videtto, Emily; Woodley, Cheryl M.; Young, Lyndsay; Drake, Richard (2025). NCCOS Assessment: Use of ‘Omics to Support Coral Interventions: Coral fertilization trials data to Optimize Sexual Reproduction in corals from Key Largo, FL from 2022-08-13 to 2022-08-17, 2023-08-04 to 2023-08-05, and 2023-09-05 (NCEI Accession 0302978). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/h4me-aw30. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license This dataset has been dedicated to the public domain under the Creative Commons CC0 1.0 Universal (CC0 1.0) Public Domain Dedication. SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) National Centers for Coastal Ocean Science, 2024: NCCOS Assessment: Use of Omics to Support Coral Interventions: Proteomics of gametes collected from Acropora cervicornis, Acropora palmata, Orbicella faveolata to Optimize Sexual Reproduction in corals from Key Largo, FL, from 2022-08-13 to 2022-08-17, 2023-08-04 to 2023-08-05, and 2023-09-05, https://www.fisheries.noaa.gov/inport/item/73852. 2024-11-12 publication gov.noaa.nmfs.inport:73852 https://www.fisheries.noaa.gov/inport/item/73852 HTTPS https://www.fisheries.noaa.gov/inport/item/73852 InPort Metadata Record information NOAA National Centers for Coastal Ocean Science (NCCOS) https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information publisher tableDigital crossReference Parsons, Emily; May, Lisa; Toline, C. Anna; Sotka, Eric; Woodley, Cheryl M. (2024). NCCOS Assessment: Fertilization cross results examining genetic relatedness impact on successful reproduction in three species of reef building corals in the Florida Reef Tract in 2019 and 2022 (NCEI Accession 0289357). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/snwd-4v72. 2024-03-07 publication International DOI Foundation (IDF) https://doi.org/10.25921/snwd-4v72 NOAA National Centers for Environmental Information gov.noaa.nodc:0289357 https://doi.org/10.25921/snwd-4v72 HTTPS https://doi.org/10.25921/snwd-4v72 Associated NCEI dataset information Parsons, Emily College of Charleston http://www.cofc.edu/ HTTP College of Charleston website Institution web page information author May, Lisa NOAA National Centers for Coastal Ocean Science https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information author Toline, C. Anna National Park Service, Region 2, Charleston, SC https://www.nps.gov HTTPS National Park Service (NPS) website Institution web page information author Sotka, Eric Graduate Program in Marine Biology, University of Charleston, Charleston, SC author Woodley, Cheryl M. NOAA National Centers for Coastal Ocean Science https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference collection eng; USA utf8 environment oceans biota -80.294217 -80.211317 25.130967 25.2248 2022-08-13 2023-09-05 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Excel PDF PNG 180.684 https://doi.org/10.25921/h4me-aw30 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/302978 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/302978 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0238/0302978/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0302978 v1.1 was published. 2025-04-04T19:13:34Z NCEI Accession 0302978 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0302978/1.1 HTTPS NCEI Accession 0302978 v1.1 published 2025-04-04T19:13:34Z download dataset Parameter or Variable: Orbicella faveolata (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: na Parameter or Variable: Acropora cervicornis (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: na Parameter or Variable: Acropora palmata (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: na Parameter or Variable: Gamete Compatibility (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: na Parameter or Variable: Coral Sexual Reproduction (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: na Parameter or Variable: Glycan Intensity (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: TIMS (2022) and Bruker Scimax (2023); Sampling and Analyzing Method: Gametes from three species of coral (Orbicella faveolata, Acropora cervicornis,and Acropora palmata) were collected across two spawning years (2022, 2023). Fertilization rates of reciprocal crosses (performed in triplicate) were compared with glycan expression of individual gametes (run in duplicate).; Data Quality Method: MALDI Imaging: Glycan peaks were identified from glycan databases, although not all peaks have been fully annotated. Parameter or Variable: Peptide Intensity (measured); Units: na; Observation Category: laboratory analysis; Sampling Instrument: Orbitrap Fusion Lumos LC-MS/MS; Sampling and Analyzing Method: Gametes from three species of coral (Orbicella faveolata, Acropora cervicornis,and Acropora palmata) were collected across two spawning years (2022, 2023). Fertilization rates of reciprocal crosses (performed in triplicate) were compared with proteomics of individual gametes.; Data Quality Method: Proteomic Analysis Peptides digested from isolated proteins were identified from annotated genomes asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian camera camera An instrument for recording images. laboratory analysis laboratory analysis use as an observation type mass spectrometer mass spectrometer Instrument that measure the massess and relative concentrations of atoms and molecules