{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Leslie Thompson", "hasEmail": "mailto:thompson.leslie@epa.gov"}, "description": "We have examined the potential for interactive cardiovascular effects of repeated, intermittent co-exposure to concentrated ambient particulate matter (CAPs) and acrolein, and the potential role of transient receptor potential cation channel A1 (TRPA1), which we previously linked to air pollution-induced cardiac arrhythmogenesis. Chemical and source characteristics of collected particles was evaluated, as well as wind and weather patterns during exposure. Female B6129 mice and Trpa1-/- mice (n=6) were exposed to filtered air (FA), CAPs (46 \u00b5g/m3 of PM2.5 approximately 160 nm diameter), Acrolein (0.42 ppm) or CAPs+Acrolein for 3hrs/day, 2days/week, for 4 weeks. Cardiac strain data, heart function and dimensions, and transmitral blood flow were investigated with echocardiography (40 MHz) before exposures, 1 day after the first exposure, and 1 day after the final exposure. Several other biological endpoints were evaluated but the key findings from ultrasound echocardiography assessments were: elapsed time between peak strain in adjacent wall segments (i.e. myocardial strain delay), a measure of myocardial dyssynchrony, increased by ~5-fold in B6129 mice after the final exposure to CAPs+Acrolein when compared to strain delay in B6129 mice exposed to FA, CAPs, or Acrolein alone, and when compared to strain delay in Trpa1-/- mice exposed to CAPs+Acrolein. There were no changes after the first exposure in any group. \n\nThis dataset is associated with the following publication:\nThompson, L., L. Walsh, B. Martin, J. Mcgee, C. Wood, K. Kovalcik, P. Pancras, N. Coates, A. Ledbetter, D. Davies, W. Cascio, M. Higuchi, M. Hazari, and A. Farraj. Ambient Particulate Matter and Acrolein Co-Exposure Increases Myocardial Dyssynchrony in Mice: Evidence for TRPA1 Involvement.   TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA,  167(2): 559-572, (2019).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Biological%20Data.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "CAPs Acrolein Biological Data.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Chamber%20and%20Weather%20Values.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "CAPs Acrolein Chamber and Weather Values.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Elemental%20Analysis.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "CAPs Acrolein Elemental Analysis.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Source%20Apportionment.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "CAPs Acrolein Source Apportionment.xlsx"}], "identifier": "https://doi.org/10.23719/1411215", "keyword": ["Ambient air quality", "Fine Particulate Matter", "TRPA1", "acrolein", "air pollution exposure", "co-exposure", "echocardiography", "heart function", "multiple pollutants", "myocardial dyssynchrony", "myocardial synchrony"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2017-12-11", "programCode": ["020:094"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1093/toxsci/kfy262"], "rights": null, "title": "Ambient Particulate Matter and Acrolein Co-Exposure Increases Myocardial Dyssynchrony in Mice via TRPA1"}