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                <gco:DateTime>2020-04-16T13:31:23Z</gco:DateTime>
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                <gco:CharacterString>Parameter or Variable: REEF AND/OR BOTTOM REGIME - PERCENT COVER (measured);
Units: cover percent;
Observation Category: in situ;
Sampling Instrument: Chain transect;
Sampling and Analyzing Method: Sessile-benthic reef communities are characterized by the continuous intercept chain-link method (as modified from Porter, 1972), following the CARICOMP (1994) protocol. This method provides information on the percent linear cover by sessile-benthic biota and other substrate categories along transects. It allows construction of reef community profiles by assignment of metric units to each substrate transition, which serves as a high precision baseline for monitoring. The chain has links of 1.42 cm long, marked every 10 links for the facilitation of counting underwater. The exact position of the chain was guided by a series of steel nails set into available hard (abiotic) substrates along transects. Individual measurements of substrate categories, as recorded from the number of chain links are sorted, added and divided by the total distance (in chain links) on each transect to calculate the cumulative percent linear cover by each substrate species and category.</gco:CharacterString>
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Units: Number of colonies;
Observation Category: in situ;
Sampling Instrument: Line transect;
Sampling and Analyzing Method: Octocorals, except for encrusting forms (e.g. Erythropodium caribaeorum, Briareum asbestinum) are counted as the number of colonies intercepted per transect, whenever any of their branches crossed the transect reference line. Hard live coral colonies under the transect line are counted and examined visually for the prevalence of apparent infectious diseases. Colonies of similar coral species growing close together and sharing attachment surfaces are counted as individual colonies if separated by a distance of 15 cm or more.</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: species abundance (measured);
Units: individuals per 30 squared meter;
Observation Category: in situ;
Sampling Instrument: Band transect;
Sampling and Analyzing Method: Demersal diurnal non-cryptic reef fish populations and motile megabenthic invertebrates are surveyed by sets of five 10 m long by 3 m wide (30 squared meters) belt-transects centered along the reference line of transects used for sessile-benthic characterizations at each reef station. Transect width was marked with flagging tape stretched and tied to weights on each transect side. Each transect is surveyed for 12 - 15 minutes depending on the complexity of the fish community on each transect. The initial one or two minutes are dedicated to the detection of elusive and/or transitory species that swim away of the “belt-transect” area as soon as they detect a diver (e.g. snappers, jacks, mackerels, groupers, hogfish, large parrotfishes, etc.). During the next three to four minutes, the diver swam over both sides of the transect area counting fishes that form schooling aggregations over the reef (e.g. Chromis spp., Clepticus spp., etc.) and other transitory species as they enter the survey area, including the wrasses (e.g. Thalassoma, Halichoeres spp.) which tend to be attracted to divers and thereby, may increase in density during the survey. A second run over both sides of transects was performed during the next four to six minutes in order to count demersal and territorial fishes (e.g. Stegastes spp., Gramma loreto, squirrelfishes, etc.) that remain within the transect area. The last two or three minutes are dedicated to counting the small gobies, echinoderms, mollusks, and crustaceans associated with coral heads and crevices on both sides of transects.</gco:CharacterString>
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Units: individuals per size class per 30 minute ASEC Survey;
Observation Category: in situ;
Sampling Instrument: Active Search Census (roving dive);
Sampling and Analyzing Method: From 2004-2013, size-frequency observations were surveyed using an Active Search Census (ASEC) technique. This is a non-random, fixed-time method designed to optimize information of the numbers of highly mobile predatory and herbivorous fish individuals present at each of the main reef habitats, providing simultaneous information on size-frequency distributions. At each reef station, the total number of individuals of each species observed within a fixed time frame of 30 minutes was registered. Individuals were actively searched for in the water column and within crevices, ledges and potentially important hiding places. For each individual sighted, a length estimate was recorded. One ASEC survey was performed at each reef station.</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: species abundance (measured);
Units: individuals per size class per 60 squared meters;
Observation Category: in situ;
Sampling Instrument: Band transects;
Sampling and Analyzing Method: Since 2015, upon completion of the 10 m long by 3 m wide belt-transect survey the diver swims along the same depth and physio-graphic reef zone for an extra 10 by 3 meters to identify fishes and megabenthic invertebrates of commercial value (snappers, groupers, hogfishes, barracuda, mackerels, sharks, lobsters and queen conch) and/or fish species that are considered important reef herbivores (parrotfishes, doctorfishes). This provides a total of 60 squared meters where a visual total length (TL) estimate (in cm) was recorded for each individual. The cephalothorax length (measurement from the tip of the rostrum to end of the thorax), also known as carapace length (CL) in cm was used to report the size of lobsters (Panulirus spp., Scyllarides spp.) within belt-transects. Queen Conch (Strombus gigas) length was reported as the total (diagonal) shell length in cm. The precision of length estimates allowed discrimination between new recruits, small juveniles, juveniles, adult and large adult size classes.</gco:CharacterString>
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Units: grams per 60 squared meters;
Observation Category: in situ;
Sampling Instrument: Band transects;
Sampling and Analyzing Method: Since 2015, the Size-frequency abundance data for the 60 squared meter band transects was compiled per species size classes made of 5cm intervals instead of the raw estimated total length (TL) reported in the annual PRCRMP reports. Once the fish and macroinvertebrates size-frequency data compilation were finished, biomass values were calculated using FishBase (ver. 02/2019) biomass calculator tools, choosing the length-weight relationship coefficients with the best geographical relation to Puerto Rico, when available. The median of each size class was used as the length value, either as fork length (FL) or total length (TL) to get biomass estimates per individual of each species size class. Then, the biomass estimates per individual of each species size class were multiplied by the number of individuals observed for that size class per. Lastly, all species size classes biomass estimates were aggregated to get the species estimated biomass per year-site-transect.</gco:CharacterString>
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Units: grams per 30 minute ASEC Survey;
Observation Category: in situ;
Sampling Instrument: Active Search Census (roving dive);
Sampling and Analyzing Method: From 2004-2013, the Size-frequency abundance data for the 30 minute Active Search Census (ASEC) survey was compiled per species size classes made of 5cm intervals instead of the raw estimated total length (TL) reported in the annual PRCRMP reports. Once the fish and macroinvertebrates size-frequency data compilation were finished, biomass values were calculated using FishBase (ver. 02/2019) biomass calculator tools, choosing the length-weight relationship coefficients with the best geographical relation to Puerto Rico, when available. The median of each size class was used as the length value, either as fork length (FL) or total length (TL) to get biomass estimates per individual of each species size class. Then, the biomass estimates per individual of each species size class were multiplied by the number of individuals observed for that size class. Lastly, all species size classes biomass estimates were aggregated to get the species estimated biomass per year-site-transect.</gco:CharacterString>
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A 1-meter chain  may be laid across a coral reef surface so that it follows the topography of the coral reef surface with descriptions of the coral made where each link touches the coral.</gco:CharacterString>
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            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>visual observation</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>not an instrument - maybe an observation type</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
    </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>