{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "accessRights": "public",
  "bureauCode": [
    "005:18"
  ],
  "contactPoint": {
    "fn": "Narrowe, Adrienne",
    "hasEmail": "mailto:adrienne.narrowe@usda.gov"
  },
  "description": "<p>The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: <a href=\"https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645\">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645</a>), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations. </p>\n<p>Resources in this dataset:</p>\n<ul>\n<li>\n<p>Resource Title: lactose sample attributes\nFile Name: lactose_sample_metadata.csv</p>\n</li>\n<li>\n<p>Resource Title: lactose SCFA, pH, and gas production data\nFile Name: lactose_SCFA_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Total bacterial and Bifidobacterium genus qPCR\nFile Name: lactose_qPCR_all_bacteria_and_bifido.csv</p>\n</li>\n</ul>",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "downloadURL": "https://ndownloader.figshare.com/files/43734918",
      "format": "csv",
      "license": "https://creativecommons.org/licenses/by/4.0/",
      "mediaType": "text/plain",
      "title": "lactose_sample_metadata.csv"
    },
    {
      "@type": "dcat:Distribution",
      "downloadURL": "https://ndownloader.figshare.com/files/43734921",
      "format": "csv",
      "license": "https://creativecommons.org/licenses/by/4.0/",
      "mediaType": "text/plain",
      "title": "lactose_SCFA_data.csv"
    },
    {
      "@type": "dcat:Distribution",
      "downloadURL": "https://ndownloader.figshare.com/files/43734930",
      "format": "csv",
      "license": "https://creativecommons.org/licenses/by/4.0/",
      "mediaType": "text/plain",
      "title": "lactose_qPCR_all_bacteria_and_bifido.csv"
    }
  ],
  "identifier": "10.15482/USDA.ADC/1529150",
  "keyword": [
    "16S rRNA sequence data",
    "ARS",
    "NP306",
    "data.gov",
    "lactose",
    "short chain fatty acids"
  ],
  "license": "https://creativecommons.org/licenses/by/4.0/",
  "modified": "2025-11-21",
  "programCode": [
    "005:040"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "Agricultural Research Service"
  },
  "spatial": [
    {
      "@type": "Location",
      "prefLabel": "{\"type\": \"Point\", \"coordinates\": [-75.187092456102, 40.078126249741]}"
    }
  ],
  "temporal": [
    {
      "@type": "PeriodOfTime",
      "endDate": "2021-06-01",
      "startDate": "2021-06-01"
    }
  ],
  "title": "Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner"
}