{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Daniel Villeneuve", "hasEmail": "mailto:villeneuve.dan@epa.gov"}, "description": "Triclocarban (TCC) is a widely used antimicrobial agent that is routinely detected in surface waters. The present study was designed to examine TCC\u2019s efficacy and mode of action as a reproductive toxicant in fish. Reproductively mature Pimephales promelas were continuously exposed to either 1 or 5 \u03bcg TCC/L,  0.5 \u03bcg 17\u03b2-trenbolone (TRB)/L or a mixture (MIX) of 5 \u03bcg TCC and 0.5 \u03bcg TRB/L for 22 d and a variety of reproductive and endocrine-related endpoints were examined. The data set includes:\n-Concentrations of the test chemicals detected in water and tissues of exposed fish\n-Ex vivo production of testosterone and estradiol by gonad tissue placed in culture (ex vivo).\n-Plasma concentrations of testosterone, 17beta estradiol, and vitellogenin\n-Targeted gene expression measurements examining relative abundance of messenger RNA coding for enzymes involved in steroid synthesis: cholesterol side-chain cleavage\n(cyp11a), 17-a-hydroxylase/17,20 lyase (cyp17), aromatase (cyp19a1a), 3b-hydroxysteroid dehydrogenase (3bhsd), 11bhydroxysteroid dehydrogenase (11bhsd), and 17b-hydroxysteroid dehydrogenase (17bhsd) as well as five additional transcripts\nmeasured included steroidogenic acute regulatory protein (star), Vtg receptor (vtgr), follicle-stimulating hormone receptor (fshr), luteinizing hormone receptor (lhr), and\nandrogen receptor (ar). \n-Ovarian transcriptomics data measured using a 15000 feature oligonucleotide microarray (GEO Platform Accession GPL10259).\n-Survival, reproduction, and morphological data.\n-. \n\nThis dataset is associated with the following publication:\nVilleneuve , D., K. Jensen , J. Cavallin , E. Durhan, N. Garcia-Reyero, M. Kahl , R. Leino, E. Makynen, L. Wehmas, E. Perkins, and G. Ankley. Effects of the anti-microbial contaminant triclocarban and co-exposure with the androgen 17\u00e2-trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas).   ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(1): :231-242, (2017).", "distribution": [{"accessURL": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64291", "title": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64291"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503126/ORD-011209%20Triclocarban%20Science%20Hub%20Data%20File.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "ORD-011209 Triclocarban Science Hub Data File.xlsx"}], "identifier": "https://doi.org/10.23719/1503126", "keyword": ["adverse outcome pathway", "aquatic ecosystems", "ecotoxicology", "endocrine disruption", "screening and prioritization"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2018-10-26", "programCode": ["020:095"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1002/etc.3531", "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110301/"], "rights": null, "title": "Effects of the antimicrobial contaminant triclocarban, and co-exposure with the androgen 17beta-trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas)."}