{
  "accessLevel": "public",
  "bureauCode": [
    "010:12"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "Jeffrey M Lorch",
    "hasEmail": "mailto:jlorch@usgs.gov"
  },
  "description": "The survival of Pseudogymnoascus destructans (Pd) was evaluated at temperatures outside of its thermal range of growth on three different artificial growth media; Sabouraud dextrose agar (SD), brain-heart infusion agar (BHI), and brain-heart infusion agar supplemented with 10% sheep red blood cells (BHI+B). Pd was harvested from starting cultures grown of MEA agar at 7˚C for 60 days. Harvested conidia were diluted in Phosphate Buffered Saline + Tween20 and spread onto plates of a given medium. Plate were then incubated at either 24, 30 or 37˚C. Plates were incubated for 1, 5, 9, 15, 30, 60, 90, 120, or 150 days before being transferred to a 7˚C incubator for 50 days. Colony forming units (CFUs) of Pd were then enumerated, resulting in a time series of Pd survival on a given medium at a given temperature. As each medium was inoculated from a different starting culture of Pd, a control group for each medium was created by inoculating plates as above and then immediate incubation at 7˚C for 50 days. The number of CFUs on the control plates was used as a statistical offset factor which allowed for the fair comparison of Pd survival between different media. The number of conidia initially plated onto each plate varied between 100, 1000 , and 10,000, depending on the temperature-medium treatment combination. In order to ensure robust statistical analysis, all data was rescaled by an appropriate correction factor which is also presented in the datafile.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "accessURL": "https://doi.org/10.5066/P9WCBGUQ",
      "description": "Landing page for access to the data",
      "format": "XML",
      "mediaType": "application/http",
      "title": "Digital Data"
    },
    {
      "@type": "dcat:Distribution",
      "description": "The metadata original format",
      "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5d14e267e4b0941bde5b749c.xml",
      "format": "XML",
      "mediaType": "text/xml",
      "title": "Original Metadata"
    }
  ],
  "identifier": "http://datainventory.doi.gov/id/dataset/USGS_5d14e267e4b0941bde5b749c",
  "keyword": [
    "Fungal pathogens",
    "Pseudogymnoascus destructans",
    "USGS:5d14e267e4b0941bde5b749c",
    "biota",
    "pathogen survival",
    "persistence",
    "temperature",
    "white-nose syndrome"
  ],
  "modified": "2020-08-21T00:00:00Z",
  "publisher": {
    "@type": "org:Organization",
    "name": "U.S. Geological Survey"
  },
  "spatial": "-89.485681, 43.048161, -89.483278, 43.050106",
  "theme": [
    "geospatial"
  ],
  "title": "Pseudogymnoascus destructans survival at elevated temperatures – Artificial media count data"
}