{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Daniel Villeneuve", "hasEmail": "mailto:villeneuve.dan@epa.gov"}, "description": "In September, 2015, a water sample was collected downstream of a major metropolitan waste water treatment plant that discharges to the South Platte River, Colorado, USA. The grab sample, 1L, was collected just below the water surface, directly into a pre-cleaned, organic-free, amber glass bottle. The water sample was extracted by solid phase extraction using an Oasis-HLB glass catridge. Cartidges were conditioned sequentially using 5mL each of ethyl acetate, 50:50 methanol (MeOH):dichloromethane (DCM), MeOH, and water. The extract in DMSO was tested in the Attagene cis- and trans-FactorialTM assays (http://www.attagene.com/technology.php; Martin and others 2010; Romanov and others 2008). Data were analyzed using an established analysis pipeline for analyzing ToxCast\u2122 high throughput screening data (Filer and others 2017). \"Active hits\" in the Attagene assay are included in the data table. \n\nThis dataset is associated with the following publication:\nKnapen, D., M. Angrish, M. Fortin, I. Katsiadaki, M. Leonard, L. Mariotta-Casaluci, S. Munn, J. O'Brien, N. Pollesch, L.C. Smith, X. Zhang, and D. Villeneuve. Adverse outcome pathway networks I: Development and applications.   ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 37(6): 1723-1733, (2018).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1423303/Attagene%20SP%202014-15_ScienceHub.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Attagene SP 2014-15_ScienceHub.xlsx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1423303/WG1-paper-1-supplementary-2018-02-08.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "WG1-paper-1-supplementary-2018-02-08.docx"}], "identifier": "https://doi.org/10.23719/1423303", "keyword": ["adverse outcome pathway", "aquatic ecosystems", "ecotoxicology", "endocrine disruption", "screening and prioritization"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2018-03-01", "programCode": ["020:095"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1002/etc.4125"], "rights": null, "title": "Adverse Outcome Pathway Networks I: Development and Applications"}