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        <gco:CharacterString>This metadata record describes the imagery collected in 2022 of Aua Reef, American Samoa by the Ecosystem Sciences Division (ESD) of NOAA, funded by the Coral Reef Conservation Program (CRCP),  to study effects of land-based source pollution by establishing biological, physical, and chemical baselines.  

In September 2022, NOAA scientists conducted benthic survey protocols at 18 study sites in Aua, American Samoa. The surveys implemented a one-stage stratified random sampling (StRS) design and the survey domain encompassed the majority of the mapped area of reef and hard bottom habitats in the 0 to 6 m depth range. Photoquadrat benthic images were collected along belt transect surveys of coral demography according to protocols established by NOAA National Coral Reef Monitoring Program (NCRMP) in 2015. 

Thirty images were collected along one, haphazardly laid, 30-m transects. Still photographs were collected to record the benthic community composition at predetermined points along belt transect with a high-resolution digital camera mounted on a pole. Photographs were taken every 1 m from the 1 m to the 30 m mark. This work generates 30 photographs per site, which are later analyzed by ESD staff and partners using the computer program CoralNet. This analysis is the basis for estimating benthic cover and composition at each site, which is documented and archived separately.
 
The StRS design effectively reduces estimate variance through stratification using environmental covariates and by sampling more sites rather than more transects per site. Therefore, site-to-site comparisons should proceed with caution.</gco:CharacterString>
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The imagery described in this metadata record is the source imagery analyzed to produce benthic cover data, which is archived and documented separately (see 'Related Items' below)</gco:CharacterString>
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            <gco:CharacterString>Hard bottom habitats including backreef, reef flat, and forereef were surveyed.

Only shallow water (0 to 30 meter depth range) hard-bottom habitats are surveyed. The survey sites were randomly selected using a systematic process based on available habitat and bathymetric data. Although efforts are made to include broad spatial coverage of surveys at each island, weather conditions and other environmental constraints may have prevented the team from surveying exposed reef areas.</gco:CharacterString>
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            <gco:CharacterString>The same methods of data collection were used at each surveyed site.</gco:CharacterString>
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            <gco:CharacterString>Benthic photographs were collected during belt transect surveys of corals by the NOAA Ecosystem Sciences Division (ESD) following the same protocol to that established by the NOAA National Coral Reef Monitoring Plan (NCRMP).

BV ADD / MENTION THE COMPLEMENTARY IMAGERY / SITES COLLECTED AND HOW THEY WERE SELECTED</gco:CharacterString>
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                <gco:CharacterString>Biological surveys implemented a modified stratified random sampling design to assess the survey domain which encompassed the hard-bottom reef habitat of Aua from 0 to 6 m in depth, and between the Aua stream mouth to the Onesosopo Park. Based on the nature and scientific questions addressed in this study, the survey domain was divided into three categorical LBSP impact sectors (15-20 Ha each) based on their distance from the source of disturbance as follows: High Impact, Medium Impact, and Low impact. Subsequently a digital map of the survey domain was overlaid with a 50 m x 50 m (2500 m2 in area) GIS layer, and grid cells containing hard-bottom reef habitat were designated as the sampling units, hereafter referred to as survey sites. Depending on the number of 50 m x 50 m grid cells fitting within, four to eight  randomly selected survey sites were allocated within each categorical impact sectors.

One, haphazardly laid, 30-m belt-transect was the focal point of the photoquadrat survey. Still photographs were collected to record the benthic community composition with a high-resolution digital camera mounted on a pole. Thirty photographs were taken every meter from the 1 m to the 30 m mark. This work generates 30 photographs per site, which are later analyzed by ESD staff and partners using the computer program CoralNet.

Survey protocols followed the National Coral Reef Monitoring Program methodologies historically implemented by NOAA's Ecosystem Sciences Division.</gco:CharacterString>
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                    <gco:CharacterString>Winston, Morgan S</gco:CharacterString>
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                    <gco:CharacterString>Ecosystem Sciences Division Standard Operating Procedures: Data Collection for Rapid Ecological Assessment Benthic Surveys, 2019 Update</gco:CharacterString>
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                        <gco:CharacterString>NOAA Pacific Islands Fisheries Science Center</gco:CharacterString>
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                                <gco:CharacterString>NOAA Technical Memorandum NMFS-PIFSC ; 96</gco:CharacterString>
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                                <gco:CharacterString>Winston W, Couch C, Huntingon B, Vargas-Ãngel B. 2020. Ecosystem Sciences Division standard operating procedures: data collection for rapid ecological assessment benthic surveys,2019 update. NOAA Tech. Memo. NMFS-PIFSC-96, 56 p</gco:CharacterString>
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