Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content

Woman skeletal muscle transcriptome with bed rest and countermeasures.

Metadata Updated: December 6, 2023

Microgravity has a dramatic impact on human physiology illustrated in particular with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for the definition of efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group n=8) and the potential beneficial impact of protein supplementation (BRN group n=8) and of a combined resistance and aerobic training (BRE group n=8). Gene expression profiles were obtained using an in-house made microarray containing 6681 muscles-relevant genes. A two-class statistical analysis was applied on the 2103 genes with consolidated expression. We identified 472 and 207 modified genes respectively for SOL and VL in BRC group. Further clustering approaches identifying relevant biological mechanisms or pathways underlined five main subclusters. Three are composed almost of upregulated genes involved mainly in nucleic acid and protein metabolism and two composed almost of downregulated genes involved in energy metabolism. Exercise countermeasure demonstrated a drastic compensatory effect decreasing the number of differentially-expressed genes by 89 and 96% in SOL and VL. In contrast nutrition countermeasure had a moderate effect and decreased the number of differentially-expressed genes by 40 and 25% in SOL and VL. Our results allowed reporting a systematic global and comprehensive view of long-term woman muscle atrophy and brought new lights and insights for space environment and for women who undergo a long-term clinical bed rest. Biological samples were collected from Pre- and Post- bed rest (BR) soleus and vastus lateralis biopsies of each subject from the three groups (bed rest only: BRC; Exercise: BRE; Nutrition: BRN). six technical replicate values (2 duplicate hybridizations a et b to chips with triplicate spots xxx) were obtained for each skeletal muscle sample. Thus for each subject 12 expression measurements (6 before BR and 6 after BR) were obtained for each muscle.

Access & Use Information

Public: This dataset is intended for public access and use. License: us-pd

Downloads & Resources


Metadata Created Date January 31, 2023
Metadata Updated Date December 6, 2023
Data Update Frequency irregular

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date January 31, 2023
Metadata Updated Date December 6, 2023
Publisher National Aeronautics and Space Administration
Identifier nasa_genelab_GLDS-51_adw8-qgze
Data First Published 2021-05-21
Data Last Modified 2023-01-26
Category Earth Science
Public Access Level public
Data Update Frequency irregular
Bureau Code 026:00
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id 6d38f646-2f9d-4085-975e-e5f041c1d591
Harvest Source Id 58f92550-7a01-4f00-b1b2-8dc953bd598f
Harvest Source Title NASA Data.json
Homepage URL
Program Code 026:005
Source Datajson Identifier True
Source Hash b33fe0970bad3f32f93015a23e2fdcfea38ff11a49954ed415e35216bfec808f
Source Schema Version 1.1

Didn't find what you're looking for? Suggest a dataset here.