In 2016, the U.S. Fish and Wildlife Service, U.S. Geological Survey, and U.S. Army Corps of Engineers undertook a large-scale interagency field study to determine the influence of commercial barge vessels on the efficacy of the Electric Dispersal Barrier System (EDBS) in the Chicago Sanitary and Ship Canal (CSSC) in preventing fish passage. This study included a series of trials in which a tow, consisting of a tug vessel and six fully-loaded barges, transited the EDBS in both upstream-bound and downstream-bound directions. The U.S. Geological Survey measured velocity profiles during these trials using synchronized velocity probes mounted on both the barge and the west canal wall. SonTek Argonaut SW 300 kHz acoustic Doppler velocity meters (SW) were used in both wall- and barge-mounted configurations, along with a wall-mounted 600 kHz Teledyne RD Instruments Channel Master horizontal acoustic Doppler current profiler (CM) and a barge-mounted 1,200 kHz CM. All velocity measurements were averaged over 10 second intervals during measurement and are included in this data release as .csv files.