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    • The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400)

    Metadata Updated: December 1, 2023

    Flow cytometry provides a rapid and reproducible method for analyzing crustacean hemocytes and their functions under experimentally-varied environmental conditions. We used flow cytometry to determine if there was a difference in hematology and selected immune functions, and intracellular pH (pHi), under two different, future ocean acidification scenarios (pH = 7.51, 7.80) compared to current conditions (pH = 8.06) for Chionoecetes bairdi, the Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water. Total counts of hemocytes did not vary among experimental control and treatments; however, there was a significantly greater number of dead, circulating hemocytes in crabs held at the lowest pH treatment. Phagocytosis of fluorescent microbeads by hemocytes was greatest at the lowest pH treatment. These results suggest that hemocytes were dying, likely by apoptosis, at a rate faster than upregulated phagocytosis was able to remove moribund cells from circulation at the lowest pH. There was no significant difference in pHi within hyalinocytes among pH levels, with apparent regulation to a mean pHi of 7.24, significantly lower than the external environment. In contrast, there was a significant difference between treatments in pHi of the semi-granular+granular cells. These findings suggest that future, predicted levels of ocean acidification may affect the defense cells of Tanner crabs, possibly making them more susceptible to other stressors in the environment.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date 2023-09-28T12:31:08Z
    Metadata Created Date December 4, 2020
    Metadata Updated Date December 1, 2023
    Reference Date(s) January 13, 2015 (publication), October 7, 2021 (revision)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NOAA/NESDIS/ncei/accessions

    Other Data Resources

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    Preview graphic

    Additional Metadata

    Resource Type Dataset
    Metadata Date 2023-09-28T12:31:08Z
    Metadata Created Date December 4, 2020
    Metadata Updated Date December 1, 2023
    Reference Date(s) January 13, 2015 (publication), October 7, 2021 (revision)
    Responsible Party (Point of Contact)
    Contact Email
    Guid gov.noaa.nodc:0123400
    Access Constraints Cite as: Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, W. Christopher; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v59884zw. Accessed [date]., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
    Bbox East Long -152.2917
    Bbox North Lat 57.7208
    Bbox South Lat 57.7208
    Bbox West Long -152.2917
    Coupled Resource
    Frequency Of Update asNeeded
    Graphic Preview Description Preview graphic
    Graphic Preview File https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0123400
    Graphic Preview Type PNG
    Harvest Object Id dd9017fc-203f-42e6-8308-83383b8db705
    Harvest Source Id c084a438-6f6b-470d-93e0-16aeddb9f513
    Harvest Source Title NOAA/NESDIS/ncei/accessions
    Licence accessLevel: Public
    Lineage
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Point", "coordinates": [-152.2917, 57.7208]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2011-07-01
    Temporal Extent End 2013-07-06

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