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Space environmental factor impacts upon murine colon microbiota and mucosal homeostasis

Metadata Updated: December 6, 2023

We report how high and low linear energy transfer (LET) radiation microgravity and elevated dietary iron affect colon microbiota (determined by 16S rDNA pyrosequencing) and colon function. Three independent experiments were conducted: 1) fractionated low LET gamma radiation (137Cs 3 Gy RAD) high Fe diet (IRON) (650 mg/kg diet) and a combination of low LET gamma radiation and high Fe diet (IRON+RAD) in male Sprague-Dawley rats; 2) high LET 38Si particle exposure (0.050 Gy) 1/6 G partial weight bearing (PWB) and a combination of high LET38Si particle exposure and PWB in female BalbC/ByJ mice; and 3) 13 d spaceflight in female C57BL/6 mice. For each experiment the colon was resected and feces removed for microbial sequencing analysis on a Roche 454 Genome Sequencer FLX Titanium instrument (Microbiome Core Facility Chapel Hill NC) using the GS FLX Titanium XLR70 sequencing reagents and protocols. Analysis of amplicon sequencing data was carried out using the QIIME pipeline. Low LET radiation high iron diet and spaceflight increased Bacteroidetes and decreased Firmicutes. Low LET radiation high Fe diet and spaceflight did not significantly affect diversity or richness or elevate pathogenic genera. Spaceflight increased Clostridiales and decreased Lactobacillales and similar trends were observed in the experiment using a ground-based model of microgravity suggesting altered gravity may affect colonic microbiota. Microbiota characteriztion in these models is a first step in understanding the impact of the space environment on intestinal health.

Access & Use Information

Public: This dataset is intended for public access and use. License: us-pd

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Dates

Metadata Created Date January 31, 2023
Metadata Updated Date December 6, 2023
Data Update Frequency irregular

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date January 31, 2023
Metadata Updated Date December 6, 2023
Publisher National Aeronautics and Space Administration
Maintainer
Identifier nasa_genelab_GLDS-72_9d67-y8yu
Data First Published 2021-05-21
Data Last Modified 2023-01-26
Category Earth Science
Public Access Level public
Data Update Frequency irregular
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 1e12f8fe-cfb6-4e40-867f-8378abe7ccc9
Harvest Source Id 58f92550-7a01-4f00-b1b2-8dc953bd598f
Harvest Source Title NASA Data.json
Homepage URL https://data.nasa.gov/d/9d67-y8yu
License http://www.usa.gov/publicdomain/label/1.0/
Program Code 026:005
Source Datajson Identifier True
Source Hash 04fd7fcd09e8a1ab2a16e6d9be2c538cc8191baa90a7673f4aa1c95b9e312989
Source Schema Version 1.1

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