Space environmental factor impacts upon murine colon microbiota and mucosal homeostasis

Metadata Updated: November 12, 2020

We report how high and low linear energy transfer (LET) radiation microgravity and elevated dietary iron affect colon microbiota (determined by 16S rDNA pyrosequencing) and colon function. Three independent experiments were conducted: 1) fractionated low LET gamma radiation (137Cs 3 Gy RAD) high Fe diet (IRON) (650 mg/kg diet) and a combination of low LET gamma radiation and high Fe diet (IRON+RAD) in male Sprague-Dawley rats; 2) high LET 38Si particle exposure (0.050 Gy) 1/6 G partial weight bearing (PWB) and a combination of high LET38Si particle exposure and PWB in female BalbC/ByJ mice; and 3) 13 d spaceflight in female C57BL/6 mice. For each experiment the colon was resected and feces removed for microbial sequencing analysis on a Roche 454 Genome Sequencer FLX Titanium instrument (Microbiome Core Facility Chapel Hill NC) using the GS FLX Titanium XLR70 sequencing reagents and protocols. Analysis of amplicon sequencing data was carried out using the QIIME pipeline. Low LET radiation high iron diet and spaceflight increased Bacteroidetes and decreased Firmicutes. Low LET radiation high Fe diet and spaceflight did not significantly affect diversity or richness or elevate pathogenic genera. Spaceflight increased Clostridiales and decreased Lactobacillales and similar trends were observed in the experiment using a ground-based model of microgravity suggesting altered gravity may affect colonic microbiota. Microbiota characteriztion in these models is a first step in understanding the impact of the space environment on intestinal health.

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Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Data Update Frequency irregular

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Publisher National Aeronautics and Space Administration
Unique Identifier Unknown
Identifier nasa_genelab_GLDS-72
Data First Published 2018-06-26
Data Last Modified 2020-01-29
Category Earth Science
Public Access Level public
Data Update Frequency irregular
Bureau Code 026:00
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Homepage URL
Program Code 026:005
Source Datajson Identifier True
Source Hash f5c051c376cc4cffb748b9b86d91d13b7543f816
Source Schema Version 1.1

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