This data release includes images and field measurements used to map salmon spawning locations along the American River near Sacramento, California, via remote sensing; the data were collected November 5-7, 2018. The purpose of this study was to develop and test a spectrally based technique for identifying salmon spawning locations, known as redds, from various types of remotely sensed data. Traditionally, redds have been mapped by eye while walking the bank or from a boat, or by an observer in an aircraft or an interpreter visually examining aerial images. The goal of this proof-of-concept investigation was to assess the potential for more efficient, objective, and automated redd mapping from conventional true color (RGB, or red/green/blue) and hyperspectral images. This child page provides redd locations mapped in the field, with their coordinates defined by surveying the redd centers with real-time kinematic (RTK) GPS receivers. The field-mapped redds were used to train and validate various image-based approaches to mapping salmon spawning locations.