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    • Sablefish GI microbiome & histology - Incorporating Gastrointestinal Microbiome Analysis into Fish Nutritional Assessments

    Metadata Updated: May 24, 2025

    Sustainable culture of sablefish (Anoplopoma fimbria) is an active research area at NWFSC, with a primary objective of rearing a human food source under healthy, economic, and sustainable conditions. Development of sustainable feed for juvenile fish through replacement of marine fish oils is key for economic and ecological viability of sablefish culture.

    This project intends to analyze the GI microbiome of juvenile sablefish, comparing three feeds (corn oil, linseed oil, BioOregon BioBrood) used in a National Marine Aquaculture Initiative (NMAI)-funded project examining the effects of substitute lipids on growth, production composition, and lipid bioconversion. Community DNA from GI mucus and luminal contents will be extracted, quantified, and quality checked. DNA will be submitted for analysis by a microbial phylogenetic microarray bearing over a million probes for classifying more than 59,000 bacterial taxa. Second Genome, a company with exclusive licensing for this phylogenetic microarray, will conduct the the analysis for classification, relative abundance, & ordination. Complementary to microbiome analysis, morphological changes in gastrointestinal tissues will be assessed for each fish by light microscopic histology (collaboration with Mark Myers). Although this is a stand-alone project, the objective is for the microbiome approach to become an integral component of finfish nutrition research. Raw & refined classification & abundance data from array analysis & histology analysis.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date May 15, 2025
    Metadata Created Date October 19, 2024
    Metadata Updated Date May 24, 2025
    Reference Date(s) June 1, 2012 (creation), May 15, 2019 (publication)
    Frequency Of Update

    Metadata Source

    Harvested from NMFS NWFSC

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Date May 15, 2025
    Metadata Created Date October 19, 2024
    Metadata Updated Date May 24, 2025
    Reference Date(s) June 1, 2012 (creation), May 15, 2019 (publication)
    Responsible Party (Point of Contact, Custodian)
    Contact Email
    Guid gov.noaa.nmfs.inport:18020
    Access Constraints Cite As: Northwest Fisheries Science Center, [Date of Access]: Sablefish GI microbiome & histology - Incorporating Gastrointestinal Microbiome Analysis into Fish Nutritional Assessments [Data Date Range], https://www.fisheries.noaa.gov/inport/item/18020., Access Constraints: NA
    Bbox East Long -122.2962
    Bbox North Lat 47.6549
    Bbox South Lat 47.6449
    Bbox West Long -122.3062
    Coupled Resource
    Frequency Of Update
    Harvest Object Id fad95b00-2237-44bf-af07-59366454bd10
    Harvest Source Id ba43549f-8268-499d-bec8-91b164cb168f
    Harvest Source Title NMFS NWFSC
    Licence NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
    Lineage sample signals are standardized before comparisons
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Polygon", "coordinates": [[[-122.3062, 47.6449], [-122.2962, 47.6449], [-122.2962, 47.6549], [-122.3062, 47.6549], [-122.3062, 47.6449]]]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2012-05-01
    Temporal Extent End 2013-04-30

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