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Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data

Published by U.S. Geological Survey | Department of the Interior | Catalog Last Checked: May 05, 2026 at 09:02 PM | Dataset Last Updated: April 28, 2022 at 12:00 AM
This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis

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