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Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data

Metadata Updated: November 20, 2025

This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

Downloads & Resources

Dates

Metadata Created Date September 13, 2025
Metadata Updated Date November 20, 2025

Metadata Source

Harvested from DOI USGS DCAT-US

Additional Metadata

Resource Type Dataset
Metadata Created Date September 13, 2025
Metadata Updated Date November 20, 2025
Publisher U.S. Geological Survey
Maintainer
Identifier http://datainventory.doi.gov/id/dataset/usgs-5d36fbfce4b01d82ce8a6cbf
Data Last Modified 2022-04-28T00:00:00Z
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://ddi.doi.gov/usgs-data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 4da4a791-634b-4cc3-8222-6db80948aefc
Harvest Source Id 2b80d118-ab3a-48ba-bd93-996bbacefac2
Harvest Source Title DOI USGS DCAT-US
Metadata Type geospatial
Source Datajson Identifier True
Source Hash 53369a502e807589743d17bf89e47bbdb825e20f64bee5fef881772b1afb7d57
Source Schema Version 1.1

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