Proteomics Core

Metadata Updated: March 8, 2017

Proteomics Core is the central resource for mass spectrometry based proteomics within the NHLBI. The Core staff help collaborators design proteomics experiments in a way that are compatible with mass spectrometry and to modify protocols if needed to ensure they will provide high quality data. An important aspect of the Core[HTML_REMOVED]s mission is to teach users new methods, techniques and instruments. The Core also continually innovates on existing technologies.Our workflows for relative protein quantitation are based on DIGE, label-free and iTRAQ approaches. We can also help investigators identify and quantify protein posttranslational modifications, including phosphorylation, nitrosylation, acetylation, ubiquitination, etc. We provide training in proper sample preparation and lead the researchers through mass spectrometric analysis to data searching and interpretation. Users have access to a variety of proteomics software platforms (Mascot, Sequest, Proteome Discoverer, Scaffold, Protein Pilot) for re-searching the data or viewing the results.In addition to helping the NHLBI investigators, we develop new approaches for characterization of posttranslational modifications and absolute protein quantitation.The NHLBI Proteomics Core was a recipient of the 2012 NHLBI Orloff Award for outstanding research support.

Recent studies and method development include:Acetylation on lysine (developing workflows for identification and occupancy measurements)Nitrosylation on cysteine (developing workflows for identification and occupancy measurements)Ubiquitination and SUMOylation (workflows for identification and quantitation)Development of [HTML_REMOVED]mito-panel[HTML_REMOVED] - using targeted analysis on Velos to follow specific mitochondrial proteinsDepletion of the most abundant serum proteins to study the protein differences in patients[HTML_REMOVED] samplesAbsolute protein quantitation using SRM on Velos instrumentsDevelopment of iTRAQ quantitation software (Quari) and measurement of and correction for isolation purity in iTRAQSeparation of peptides and phosphopeptide enrichment using IEF or OFF-Gel SystemInstrumentsMass spectrometers Thermo Orbitrap Velos Elite 1

Thermo Orbitrap Velos Elite 2

Thermo Orbitrap Excel with ETD

ABI 5800 MALDI-TOF/TOF

ABI 4000 Qtrap2D-gel setup Typhoon Scanner

Ettan Spot Handling Workstation

Ettan Spot Picker

Perkin Elmer Liquid Handling Station

Progenesis software programsSupporting instruments Three off-line Agilent HPLC for SCX/HILIC

OFFGEL Instrument for IEF separation of peptides and proteins

GelFree Instrument for in-solution separation of proteinsData analysis Workstations with Proteome Discoverer, Scaffold, Protein Pilot, Mascot Daemon, ProteoIQ

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date March 8, 2017
Metadata Updated Date March 8, 2017

Metadata Source

Harvested from Federal Laboratory Consortium Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date March 8, 2017
Metadata Updated Date March 8, 2017
Publisher Federal Laboratory Consortium
Unique Identifier 9289BC2F-0CD8-4720-B968-95C287B0B252
Maintainer
Marjan Gucek
Maintainer Email
Public Access Level public
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Schema Version https://project-open-data.cio.gov/v1.1/schema
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Flcbusinessurl https://flcbusiness.federallabs.org/#/laboratory/5300
Harvest Object Id 745ac05e-6413-4501-ab98-671b7ba16c19
Harvest Source Id 5859cfed-553c-48de-a478-b67b5225f6fc
Harvest Source Title Federal Laboratory Consortium Data.json
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Repphone (301) 594-1060
Sizesqft 0
Source Datajson Identifier True
Source Hash 34cc604d9b1e8508cf10c2448be67a8cf2538273
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