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    • Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077)

    Metadata Updated: November 1, 2023

    In this work, I took a protein profiling approach known as iTRAQ approach to characterize and quantify the proteins synthesized by 12 experimental coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu"; 21°56’18.01’’N, 120°44’45.54’’E) and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan"; 22°01'23.30"N, 120°41'18.29"E) and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML (open-access mass spectrometry data) files (SHVTS-A.mzml & SHVTS-B.mzml), two MZID files (SHVTS-A.mzid & SHVTS-B.mzid), and a tab-delineated file that describes which samples were labeled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome described in Mayfield et al. (2016 Mol Ecol) and found here: http://symbiont.iis.sinica.edu.tw/s_hystrix/static/html/#stat. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific).

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date 2023-05-14T12:24:25Z
    Metadata Created Date June 4, 2021
    Metadata Updated Date November 1, 2023
    Reference Date(s) September 9, 2020 (publication)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NOAA/NESDIS/ncei/accessions

    Other Data Resources

    Graphic Preview

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    Additional Metadata

    Resource Type Dataset
    Metadata Date 2023-05-14T12:24:25Z
    Metadata Created Date June 4, 2021
    Metadata Updated Date November 1, 2023
    Reference Date(s) September 9, 2020 (publication)
    Responsible Party (Point of Contact)
    Contact Email
    Guid gov.noaa.nodc:0216077
    Access Constraints Cite as: Mayfield, Anderson B.; Cooperative Institute for Marine and Atmospheric Studies, University of Miami; Atlantic Oceanographic and Meteorological Laboratory (AOML) (2020). Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0216077. Accessed [date]., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
    Bbox East Long 120.74598
    Bbox North Lat 22.02314
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    Bbox West Long 120.68841
    Coupled Resource
    Frequency Of Update asNeeded
    Graphic Preview Description Preview graphic
    Graphic Preview File https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0216077
    Graphic Preview Type PNG
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    Harvest Source Id c084a438-6f6b-470d-93e0-16aeddb9f513
    Harvest Source Title NOAA/NESDIS/ncei/accessions
    Licence accessLevel: Public
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    Metadata Language eng
    Metadata Type geospatial
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    Progress completed
    Spatial Data Service Type
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    Spatial Harvester True
    Temporal Extent Begin 2010-05-14
    Temporal Extent End 2010-06-15

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