Non-Destructive Detection and Separation of Radiation Damaged Cells in Miniaturized, Inexpensive Device, Phase I

Metadata Updated: May 2, 2019

There is a clear and well-identified need for rapid, efficient, non-destructive detection and isolation of radiation damaged cells. Available commercial technologies are expensive, require core facilities and use destructive methods. We propose to develop and demonstrate a novel fully automated, microfluidics-based device for identification and sorting of radiation damaged cells. The final product will be simple, small, inexpensive and fieldable in research environments as well as space. We will identify novel cell surface markers indicating radiation damage using a microarray (gene expression) experiments and verify downselected markers (protein upregulation) using fluorescent antibody tagged microparticles. CFDRC's proprietary dielectrophoretic cell sorter technology will be adapted for automated separation of the tagged damaged cells from overall population of cells. Proof-of-concept will be demonstrated by separation of damaged cells from an irradiated cell sample. Phase II efforts will focus along two primary lines. Surface biomarkers discovery will be further extended and validated. An integrated microfluidic cartridge and instrumentation capable of all operations (storage, mixing, sorting) will be developed. The prototype instrument will be demonstrated with both terrestrial and space radiation (in collaboration with NASA researchers/facilities). A multi-disciplinary team consisting of experts in microfluidics engineers (CFDRC) and radiation biologists (Temple University) has been assembled.

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Public: This dataset is intended for public access and use. License: U.S. Government Work

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Dates

Metadata Created Date August 1, 2018
Metadata Updated Date May 2, 2019

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date August 1, 2018
Metadata Updated Date May 2, 2019
Publisher Space Technology Mission Directorate
Unique Identifier TECHPORT_10066
Maintainer
TECHPORT SUPPORT
Maintainer Email
Public Access Level public
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Datagov Dedupe Retained 20190501230127
Harvest Object Id 56e5fdfe-c5b7-4943-a98b-f92d8549cf87
Harvest Source Id 39e4ad2a-47ca-4507-8258-852babd0fd99
Harvest Source Title NASA Data.json
Data First Published 2008-01-01
Homepage URL https://techport.nasa.gov/view/10066
License http://www.usa.gov/publicdomain/label/1.0/
Data Last Modified 2018-07-19
Program Code 026:027
Source Datajson Identifier True
Source Hash 53ce9554d46c21df3ef477ba493df04e05245f39
Source Schema Version 1.1

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