Microbiomes of the Dust Particles Collected from the International Space Station and Spacecraft Assembly Facilities

Metadata Updated: February 28, 2019

The safety of the International Space Station (ISS) crewmembers and maintenance of ISS hardware are the primary rationale for monitoring microorganisms in this closed habitat. The composition of the microbial community of this built environment is unique due to microgravity space radiation and elevated carbon dioxide levels. As built environments are known to have their own microbiomes next-generation sequencing methods have to be utilized to explore the ISS microbial profile and use this data for further development of safety and maintenance practices. ISS vacuum cleaner bag components (surface) and high-efficiency particulate arrestance (HEPA) filter element (air) samples were analyzed by traditional cultivation adenosine triphosphate (ATP) and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR) assays to estimate viable microbial populations. In addition vacuum cleaner bag components of two cleanrooms at Jet Propulsion Laboratory (JPL Pasadena CA) were examined concurrently. The 16S rRNA gene sequencing based on Illumina platform was used to elucidate the ISS microbial diversity and explore differences between the microbiomes of the ISS and Earth-based cleanrooms. The statistical analyses of these microbiomes show that Actinobacteria Firmicutes and Proteobacteria dominate in the air and surface of the ISS and the cleanroom samples but vary in abundance. While members of Actinobacteria were predominant in the ISS Proteobacteria the least abundant phylum in the ISS dominated the Earth-based cleanrooms. The viable bacterial population (PMA-treated samples) decreased significantly but the treatment did not appear to have an effect on the bacterial composition (diversity) associated with a sampling site. Viable fungal sequences were not retrieved from the ISS HEPA sample where as highest viable fungal diversity was observed in the Earth-based cleanroom (JPL class 100K) debris. The results of this study provided strong evidence of substantial contribution of human skin-associated microorganisms such as Corynebacterium/Propionibacterium (Actinobacteria),not Staphylococcus (Firmicutes) species as the dominant species in the ISS in terms of viable and total bacterial community structure.

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Public: This dataset is intended for public access and use. License: U.S. Government Work

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Dates

Metadata Created Date August 1, 2018
Metadata Updated Date February 28, 2019
Data Update Frequency irregular

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date August 1, 2018
Metadata Updated Date February 28, 2019
Publisher National Aeronautics and Space Administration
Unique Identifier nasa_genelab_GLDS-26
Maintainer
GeneLab Outreach
Maintainer Email
Public Access Level public
Data Update Frequency irregular
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id b12ba2cc-f133-4c67-838d-de9756ea6694
Harvest Source Id 39e4ad2a-47ca-4507-8258-852babd0fd99
Harvest Source Title NASA Data.json
Data First Published 2018-06-26
Homepage URL https://data.nasa.gov/d/y6cx-abd3
License http://www.usa.gov/publicdomain/label/1.0/
Data Last Modified 2018-07-19
Program Code 026:005
Source Datajson Identifier True
Source Hash 920c60b9763e97a19701c1f714a8f1f5da9c8a19
Source Schema Version 1.1
Category Earth Science

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