We examined habitat use and foraging activity of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus), as well as nocturnal aerial insect abundance at Kaloko-Honōkohau National Historical Park located in the coastal region of Kailua-Kona, Hawai‘i Island. The study area covers approximately 486 ha of marine waters and terrestrial coastal plains. Hawaiian hoary bat echolocation vocalizations were recorded at seven acoustic stations (KAHO1-KAHO7) between 1 and 10 m above sea level, that operated nightly from November 2013 through February 2015. Nocturnal aerial insect collection occurred in January and April (2 or 3 nights each) and in July and November (1 or 2 nights each) in 2014. Insects were collected at three stations KAHO1, KAHO3, and KAHO4. These data summarize the seasonal presence of the Hawaiian hoary bat and nocturnal, aerial insect prey at Kaloko-Honokohau National Historical Park over a 15-month period from November 2013 to February 2015. This data release consists of three tabular data sets including bat acoustic activity, collected insect counts, and collected insect dry biomass data.