Toxicological data generated for Apis mellifera honey bees and an ectoparasitic mite (Varroa destructor) in laboratory toxicity trials are presented. Data were generated over a four-year period by members of the Varroacide Research and Testing Team (VRTT) as part of a foundational grant from the Foundation for Food and Agricultural Research (FFAR). Data may be used to explore toxicological responses of honey bees and Varroa mites to a wide range of natural and synthetic chemical treatments against Varroa infestations in honey bee colonies. Data may be collected and analyzed together with other data sets not generated by VRTT. Resources in this dataset:Resource Title: Toxicity data generated from vial and jar assays by USDA-ARS Beltsville bee lab in summer, 2019. File Name: Vial & Jar Data Summer 2019 USDA .csvResource Description: Toxicity data generated for Apis mellifera honey bees and Varrroa destructor mites from laboratory vial and jar assays in 2019 by the USDA-ARS Bee Research Lab, Beltsville MDResource Title: Honey bee (Apis mellifera) and Varroa destructor toxicity data from 2019 cage trials at USDA laboratory. File Name: Cage Data Summer 2019 USDA ultimate.csvResource Description: Bees and mites were housed together in 1 L plastic deli containers modified into cages. Toxicants were applied to acetate strips hung from the center of the upper lid. Bee and mite mortality were gauged at 3 time points, 4 hours, 24 hours, and 48 hours after exposure to toxicants.Resource Title: Vial and Jar toxicity data 2018 USDA. File Name: Vial Jar toxicity data Summer 2018_USDA.csvResource Description: Honey bee (Apis mellifera) and Varroa destructor mite toxicity data from vial and jar assays 2018 USDAResource Title: Vial and Jar toxicity data OSU 2018. File Name: Vial and Jar toxicity data OSU 2018.csvResource Description: Toxicity data for Varroa destructor mites and Apis mellifera honey bees completed for various active ingredients conducted by laboratory of Dr. Reed Johnson at the Ohio State University in 2018.Resource Title: Vial and Jar toxicity data OSU 2019. File Name: Vial and Jar toxicity data OSU 2019.csvResource Description: Toxicity data for Varroa destructor mites and Apis mellifera honey bees completed for various active ingredients conducted by laboratory of Dr. Reed Johnson at the Ohio State University in 2019.